滇产小百部化学成分研究  

Studies on the chemical constituents of asparagus filicinus produced in yunnan

作　　者：付欢 王家鹏 朱丽 蔡乐[2] 杨明惠 FU Huan;WANG Jia-peng;ZHU Li;CAI Le;YANG Ming-hui((1.College of pharmacy and chemistry,Dali university,yunnan key laboratory of insect biomedical research and development,Dali 671000,China;Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province,Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education,School of Chemical Science and Technology,Yunnan University,Kunming 650091,China).)

机构地区：[1]大理大学药学与化学学院,云南省昆虫生物医药研发重点实验室,云南大理671000 [2]云南大学云南省高校功能分子分析与生物转化重点实验室,教育部自然资源药物化学重点实验室,化学科学与工程学院,云南昆明650091

出　　处：《化学研究与应用》2020年第4期637-644,共8页Chemical Research and Application

基　　金：长江学者和创新团队发展计划项目(IRT_17R94)资助;云南省中青年学术和技术带头人后备人才培育项目资助;云南大学青年英才培育项目资助。

摘　　要：采用硅胶柱色谱、Sephadex LH-20柱层析、ODS反相柱色谱和制备型高效液相色谱等方法对滇产小百部的甲醇提取物进行分离纯化,并通过1H NMR、13C NMR、MS等波谱学技术对所分离化合物进行结构鉴定。从小百部中共分离鉴定了14个化合物:2-deoxy-20-hydroxyecdysone(1),20-hydroxyecdysone(2),ajugasterone C(3),ajugasterone C-2-ethyl ester(4),calonysterone(5),2-deoxyecdysone(6),ergosterol(7),胡萝卜苷(8),小百部苷A(9),小百部苷B(10),filiasparoside B(11),filiasparoside C(12),filiasparoside D(13),asparagusin A(14)。研究表明小百部中主要以甾酮和甾体皂苷类化合物为主,其中化合物1、3、4、6、7为首次从小百部中分离得到。Silica gel column chromatography,Sephadex LH-20 column chromatography,ODS reversed phase column chromatography and prepared high performance liquid chromatography were applied to separate and purify the compounds from the methanol extract of Asparagus filicinus.1H-NMR,13C-NMR and MS were adopted to determine the structures of the isolated compounds.Fourteen compounds were isolated and their structures were elucidated as:2-deoxy-20-hydroxyecdysone(1),20-hydroxyecdysone(2),ajugasterone C(3),ajugasterone C-2-ethyl ester(4),calonysterone(5),2-deoxyecdysone(6),ergosterol(7),daucosterol(8),aspafiliosides A(9),aspafiliosides B(10),filiasparoside B(11),filiasparoside C(12),filiasparoside D(13),asparagusin A(14).The results showed that sterones and steroid saponins were the predominant constituents in Asparagus filicinus.Compounds 1,3,4,6,7 were isolated from this plant for the first time.

关 键 词：小百部 甾酮 甾体皂苷 

分 类 号：O629.2[理学—有机化学]