鸭源大肠杆菌分离鉴定及其毒力基因检测  

Isolation of duck-derived pathogenic Escherichia.coli and test of virulence genes

作　　者：李迎晓[1] 焦凤超[1] 张李敏 杨露露 李洵[1] 刘锦妮[1] 吴海港[1] 黄立[1] 赵聘[1] LI Yingxiao;JIAO Fengchao;ZHANG Limin;YANG Lulu;LI Xun;LIU Jinni;WU Haigang;HUANG Li;ZHAO Pin(College of Animal Science and Veterinary Medicine/Engineering and Technology Research Center for Waterfowl Resources Development and Utilization and Epidemic Disease Prevention and Control of Henan Province,Xinyang Agricultural and Forestry University,Xinyang 464000,China)

机构地区：[1]信阳农林学院/河南省水禽资源开发利用与疫病防控工程技术研究中心,河南信阳464000

出　　处：《畜牧与兽医》2020年第3期73-77,共5页Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目:水禽常见致病菌快速检测及其耐药机制研究(182102110123);信阳农林学院动物检疫科技服务团队。

摘　　要：从信阳市部分规模化鸭场疑似大肠杆菌感染病料中,通过传统分离培养及phoA基因检测,分离得到19株大肠杆菌。对分离菌进行种系发育分群、动物致病性试验和毒力基因检测。系统发育分子分群结果显示,19株分离菌中10株(52.63%)属于B2群,7株(36.84%)属于D群,1株(5.26%)属于B1群,1株(5.26%)属于A群。动物致病性试验结果显示,高致病性菌株9株(47.38%),中致病性菌株7株(36.84%),低致病性菌株3株(15.78%)。毒力基因的检测结果显示,ompT、iroN、hlyF、iutA和Iss五种毒力基因的检出率均为89.47%;fimC、marA和irp2基因检出率为63.16%;fyuA基因检出率为42.11%。In this study,19 strains,marked as DE1~DE19,were isolated from sick ducks at farms in Xinyang City,and were identified as Escherichia coli,based on traditional methods of isolation,identification and biochemical test;and phoA genes were amplified using PCR.Rapid phylogenetic grouping technique,based on PCR,was adopted to analyze the main phylogenetic groups of the isolates.The results were that the B2 and D phylogenetic groups were the most common strains,accounting for 52.63%and 36.84%,respectively,of the of 19 isolates,and B1 and A accounted for 5.26%and 5.26%,respectively.The pathogenicity of the 19 isolates from the susceptible one-day-old ducks showed that 47.38%,36.84%and 15.78%of these isolates were characterized as of high,intermediate and low pathogenicity,respectively.The PCR test of the virulence associated genes showed that ompT,iroN,hlyF,iutA and Iss were positive for 89.47%of all the isolates;fimC,marA and irp2 were 63.16%,and fyuA was 42.11%.

关 键 词：禽致病性大肠杆菌 鸭 分离鉴定 致病性 毒力基因 

分 类 号：S855.1[农业科学—临床兽医学]