猪的免疫球蛋白基因鉴定和注释  

Identification and annotation of the porcine immunoglobulin genes

作　　者：张璞[1] 林育 游斌杰[2,3,4] 孙静 ZHANG Pu;LIN Yu;YOU Binjie;SUN Jing(Provincial Key Laboratory for Farm Animal Genetic Resource Exploration and Innovation,Sichuan Agricultural University,Sichuan 611130,China;Chongqing Academy of Animal Sciences,Chongqing 402460;Key Laboratory of Pig Industry Science of Agriculture Ministry,Chongqing 402460;Chongqing Key Laboratory of Pig Industry Science,Chongqing 402460;Technical Engineering Center for the Development and Utilization of Medical Animal Resources,Chongqing 402460)

机构地区：[1]四川农业大学畜禽品种资源发掘与利用省重点实验室,四川611130 [2]重庆市畜牧科学院,重庆402460 [3]农业部养猪科学重点实验室,重庆402460 [4]养猪科学重庆市市级重点实验室,重庆402460 [5]重庆市医用动物资源开发与利用工程技术研究中心,重庆402460

出　　处：《中国实验动物学报》2020年第2期208-215,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：重庆市科研院所绩效激励引导专项项目(cstc2018jxjl0083,cstc2018jxjl0043);重庆市农发资金(17407)。

摘　　要：目的本研究旨在鉴定猪的免疫球蛋白基因,为研究猪对疾病的抗性奠定坚实的基础,同时对生猪养殖及其作为医学模式动物都有一定意义。方法使用Blast+,R,DNAstar,WebLogo等软件通过人免疫球蛋白基因信息对猪的免疫球蛋白基因进行预测分类。结果共鉴定到猪的免疫球蛋白重链(IGH)基因43个,分为7个基因家族;免疫球蛋白κ轻链(IGK)基因27个,分为6个基因家族;免疫球蛋白λ轻链(IGL)基因25个,分为9个基因家族。结论我们发现了43个猪免疫球蛋白重链基因,其中可能存在假基因。同时猪免疫球蛋白两种轻链基因比例接近,这可能与其免疫特性有关,但仍需要相关的试验数据来开展研究。Objective The aim of this study was to establish a basis for the identification of porcine immunoglobulin genes in order to facilitate research on swine disease resistance.Further improvements of immunoglobulin gene annotation will facilitate the development of the pig industry and will promote the wider application of pigs as a major biomedical model.Methods Porcine immunoglobulin genes were predicted and classified by comparison with human immunoglobulin genes using R,DNAstar,WebLogo,and Blast+.Results A total of 43 IGH,27 IGK,and 25 IGL genes were identified in pigs,which could be divided into 7,6,and 9 gene families,respectively.Conclusions We found 43 immunoglobulin heavy chain genes in pig.Some of them may be pseudogene.Meanwhile,the ratio of light chain genes(IGK∶IGL)is approximately 1∶1.It may be related to the immunological characteristics of pig,which need related experimental data to do its research.

关 键 词：猪 免疫球蛋白 基因组 

分 类 号：Q95-33[生物学—动物学]