不同年龄段小鼠抑郁及焦虑样行为的比较  

Comparison of depression-like and anxiety-like behaviors in mice of different ages

作　　者：杨颖雯 周瑾 黄菲 YANG Yingwen;ZHOU Jin;HUANG Fei(Shanghai R&D Center for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203)

机构地区：[1]上海中医药大学中药研究所,上海中药标准化研究中心,上海201203

出　　处：《中国实验动物学报》2020年第2期248-253,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81703734);上海市青年科技英才扬帆计划(17YF1417700);上海市自然科学基金(17ZR1430200);上海市卫计委临床专项(20184Y0177);上海中医药大学大学生创新创业项目。

摘　　要：目的观察和比较不同年龄段实验小鼠的抑郁及焦虑样行为。方法将27只雄性C57BL/6小鼠按年龄分为青年组(3月龄,n=9)、中年组(10月龄,n=9)、老年组(18月龄,n=9),分别进行悬尾测试、强迫游泳测试、高架十字迷宫测试、开放旷场测试和糖水偏好测试,观察小鼠抑郁及焦虑样行为,并用液质联用(LC-MS/MS)法检测C57BL/6小鼠血清5-羟色胺含量。结果中年组小鼠比老年组小鼠的悬尾不动时间长(P<0.05);青年组、中年组、老年组小鼠强迫游泳不动时间显著递减,青年组小鼠游泳不动时间显著大于中年组小鼠(P<0.001)和老年组小鼠(P<0.001),中年组小鼠游泳不动时间显著大于老年组小鼠(P<0.01);中年组小鼠比老年组小鼠12小时糖水偏好率高(P<0.05),青年组小鼠比老年组小鼠12、36、48、60小时糖水偏好率高(P<0.05);老年组小鼠比中年组和青年组小鼠开臂时间长(P<0.05),青年组小鼠比中年组小鼠探头次数多(P<0.01);青年组小鼠比老年组小鼠在旷场中的移动总距离长(P<0.05);老年组、中年组、青年组小鼠血清5-HT含量呈递减趋势,青年组小鼠血清5-HT显著低于中年组(P<0.05)和老年组(P<0.01)。结论整体而言,同一刺激下,小鼠的年龄越小,越易表现出抑郁及焦虑样行为,血清中5-HT水平越低。Objective To observe and compare the depression-like and anxiety-like behaviors in mice of different ages.Methods Twenty-seven male C57BL/6 mice were divided into three groups according to age:young group(3 months,n=9),middle-aged group(10 months,n=9)and old group(18 months,n=9).The tail suspension test,forced swimming test,elevated plus maze test,open field test,and sugar preference test were used to detect depression-like and anxiety-like behaviors.Liquid Chromatography with tandem mass spectrometry was used to detect 5-hydroxytryptamine(5-HT)levels in the serum of mice.Results The immobility time of middle-aged mice was longer than that of old mice in tail suspension test(P<0.05);the immobility time of forced swimming decreased from young to middle-aged mice(P<0.001)and from middle-aged to old mice(P<0.01).The sucrose preference of middle-aged mice was higher than that of old mice at 12 hours(P<0.05);the sugar preference rate of young mice was higher than that of old mice at 12,36,48,and 60 hours(P<0.05).The time spent in the open arms by old mice was longer than that of middle-aged and young mice(P<0.05)and the probe number of young mice was greater than that of middle-aged mice(P<0.01).The total distance travelled in the open field by young mice was longer than that of old mice(P<0.05).The concentration of 5-HT in the serum of old,middle-aged,and young mice showed a decreasing trend.The concentration of serum 5-HT in young mice was significantly lower than that in middle-aged(P<0.05)and old mice(P<0.01).Conclusions The younger the mice,the more likely they were to exhibit depression-and anxiety-like behaviors under the same stress stimulations,and to have a lower level of 5-HT in their serum.

关 键 词：年龄段 抑郁样行为、焦虑样行为 5-羟色胺 

分 类 号：Q95-33[生物学—动物学]