桂枝甘草汤含药血清对豚鼠心室肌细胞钠通道的影响  

Effect of Guizhi Gancao Decoction Containing Serum on Sodium Channel of Guinea Pig Ventricular Myocytes

作　　者：刘俊红[1] 邢作英[1] 宋欢欢[1] 安宜沛 王永霞[1] LIU Junhong;XING Zuoying;SONG Huanhuan;AN Yipei;WANG Yongxia(The First Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou Henan China 450000)

机构地区：[1]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中医学报》2020年第4期837-839,共3页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81102698,81803944);河南省2018年科技发展计划项目(182102311152)。

摘　　要：目的:观察桂枝甘草汤及不同组分含药血清对单个豚鼠心室肌细胞钠离子通道电流及其动力学参数的影响。方法:制备桂枝甘草汤及各组分含药血清,急性分离豚鼠心室肌细胞,细胞复钙后随机分为空白组、甘草组、桂枝组、甘草加桂枝组和桂枝甘草汤组,各组分别加入各含药血清(10%、15%体积分数),加药完成后细胞放于37℃,5%CO2的孵育箱中孵育24 h后进行膜片钳实验。结果:与空白组比较,各组不同体积分数均可显著抑制Na+电流平均电流密度(P<0.05)。结论:桂枝甘草汤及各组分含药血清对Na+通道有不同程度的抑制作用,与I类抗心律失常药物作用机制相同,这可能是桂枝甘草汤抗心律失常的作用机制。Objective: To observe the effect of Guizhi Gancao Decoction and the serum containing medicine of different components on sodium channel current and kinetic parameters of single guinea pig ventricular myocytes. Methods: Guizhi Gancao Decoction and the serum containing medicine of each component were prepared,and the ventricular myocytes of guinea pig were separated acutely. The cells were randomly divided into blank group,Gancao group,Guizhi group,Gancao plus Guizhi group and Guizhi Gancao Decoction group after calcium recovery. Each group was added with each serum containing medicine( 10% and 15% volume fraction group),the cells were incubated in 37 ℃,and patch clamp test was carried out after incubation in 5% CO2 incubator for24 hours. Results: Compared with the blank group,the volume fraction of different volume fraction in each group could significantly inhibit the average current density of Na+current( P < 0. 05). Conclusion: Guizhi Gancao Decoction and the serum containing medicine of each component have different degrees of inhibition on Na+channel. The mechanism of action is the same as that of class I antiarrhythmic medicine,which may be the mechanism of action of Guizhi Gancao Decoction on antiarrhythmia.

关 键 词：桂枝甘草汤 膜片钳 钠离子电流 动力学参数 豚鼠 

分 类 号：H285.5[语言文字—少数民族语言]