基于静脉造影的静脉栓塞分级评价体系在溶栓药物治疗大鼠静脉血栓的应用研究  

Application of grading evaluation system on thrombolytic drugs in treatment of venous thrombosis in rats based on venography

作　　者：王健 孙瑜[2] 徐向伟[3] 孙双勇[3] 王维亭[3] WANG Jian;SUN Yu;XU Xiang-Wei;SUN Shuang-Yong;WANG Wei-Ting(Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Tianjin Key Laboratory of Cancer Prevention and Therapy,Tianjin’s Clinical Research Center for Cancer,Tianjin 300060,China;Tianjin University of TCM,Tianjin 300193,China;Tianjin Institute of Pharmaceutical Research New Drug Evaluation Co.Ltd,Tianjin 300301,China)

机构地区：[1]天津医科大学肿瘤医院国家肿瘤临床医学研究中心天津市“肿瘤防治”重点实验室天津市恶性肿瘤临床医学研究中心,天津300060 [2]天津中医药大学,天津300193 [3]天津药物研究院新药评价有限公司,天津300301

出　　处：《现代药物与临床》2020年第2期212-215,共4页Drugs & Clinic

基　　金：天津市科技支撑重点项目(19YFZCSY00620)。

摘　　要：目的建立一种用于大鼠静脉血栓模型的静脉栓塞分级评价体系,为溶栓药物用于临床的适应症提供参考。方法雄性Wistar大鼠制备静脉血栓。在血栓形成后30min开始给药,采用尿激酶局部输注41、82、164kU/kg及阿替普酶2.05、4.10、8.20 mg/kg静脉血栓进行治疗,给药后3 h采用血管造影法拍摄静脉栓塞部位的X光片,对静脉栓塞进行分级研究。结果尿激酶、阿替普酶均产生剂量相关性溶栓作用,5级研究体系的研究结果与血管再通率,血管通过率、血栓质量的结果一致。5级评分标准,级距设计合理,在血栓残留量、血管通过率方面显示出明显的级间差别。结论基于静脉造影的静脉栓塞分级评价体系可客观表现静脉栓塞程度,可用于评估溶栓药物静脉溶栓效果。Objective To establish a system for grading evaluation system for venous thrombosis in rats, and to provide a reference for clinical indications of thrombolytic drugs. Methods Venous thrombosis models were made in male Wistar rats. Urokinase local infusion of 41, 82, 164 kU/kg, and ateplase of 2.05, 4.10, and 8.20 mg/kg at 30 min after thrombosis were used to treat venous thrombosis. After be treated for 3 h, the X-ray film of the site of venous embolism was taken by angiography to study the grade of venous embolism. Results Urokinase and ateplase produced a dose-dependent thrombolytic effect. Grade 5 system was consistent with those of recanalization rate, vascular throughput rate, and thrombus weight. The design of grade 5 was reasonable, and there were significant differences in thrombus residue and vascular throughput rate between each grade. Conclusion The grading evaluation system of venous embolism based on venography can objectively show the degree of venous embolism, and can be used to evaluate the thrombolytic effect of thrombolytic drugs.

关 键 词：尿激酶 阿替普酶 静脉血栓 静脉栓塞分级评价体系 溶栓效果 

分 类 号：R966[医药卫生—药理学]