24个Y-STR基因座在云南苗族人群中的序列多态性  

Sequence Polymorphism of 24 Y-STR Loci among Miao-ethnic Population in Yunnan Province

作　　者：杨凯润 郭立亮 吴浩[2,3] 刘宗伟 刘婵 赵光斌 王梓齐 聂胜洁 王乐[2,3] 吴坚 YANG Kairun;GUO Liliang;WU Hao;LIU Zongwei;LIU Chan;ZHAO Guangbin;WANG Ziqi;NIE Shengjie;WANG Le;WU Jian(School of Forensic Medicine,Kunming Medical University,Kunming 650500,China;Institute of Forensic Science,Ministry of Public Security(MPS)&National Engineering Laboratory for Forensic Science,Beijing 100038,China;Institute of Forensic Science,MPS&MPS’Key Laboratory of Forensic Genetics,Beijing 100038,China;Changshu Public Security Bureau,Changshu 215500,Jiangsu,China)

机构地区：[1]昆明医科大学法医学院,昆明650500 [2]公安部物证鉴定中心现场物证溯源技术国家工程实验室,北京100038 [3]公安部物证鉴定中心法医遗传学公安部重点实验室,北京100038 [4]江苏省常熟市公安局,江苏常熟215500

出　　处：《刑事技术》2020年第2期148-154,共7页Forensic Science and Technology

基　　金：国家自然科学基金项目(81601649);公安部技术研究计划重点项目(2019JSYJA05);法医遗传学公安部重点实验室开放课题(2018FGKFKT03);中央级公益性科研院所基本科研业务费专项资金项目(2018JB007、2019JB009、2019JB045);昆明医科大学科技创新团队(CXTD201803)。

摘　　要：目的研究法医学常用Y-STR基因座在中国云南苗族男性个体中的序列多态性。方法采用M48磁珠提取纯化试剂盒提取样本DNA,使用ForenSeqTM DNA Signature Prep试剂盒制备文库,Miseq FGx平台进行测序,ForenSeq Universal Analysis v1.2.1软件进行数据分析,用Arlequin v3.5软件计算各Y-STR基因座相关的统计学参数,将Y-STR基因座长度多态性与序列多态性进行比较。结果108名云南苗族个体中共检出106种单倍型,总体单倍型多样性(HD)和Y-STR分型系统的分辨能力(DC)分别为0.9993和0.9815。24个Y-STR基因座共检出204个基因,基因多样性(gene diversity,GD)值为0.2177~0.9481,15个Y-STR基因座的GD值大于0.6。DYF387S1、DYS390、DYS389II、DYS437、DYS438、DYS448、DYS612基因座存在长度相同的基因核心序列不同的情况。结论该24个Y-STR基因座在云南苗族男性人群中具有丰富的遗传多态性。研究结果可为Y-STR数据库的建立、群体遗传学和法医学实践提供参考。Objective To investigate the sequence polymorphism of 24 Y-STR among Miao-ethnic male individuals in Yunnan province.Methods From 108 unrelated males of Yunnan Miao-ethnic population,DNA was respectively extracted with MagAttract M48 DNA Manual kit.Y-STR-specific libraries were constructed through ForenSeqTM DNA Signature Prep kit,and sequenced into the Miseq FGx platform.Sequence data were analyzed with ForenSeq Universal Analysis v1.2.1 software,and calculated of their Y-STR-locus-related statistical parameters via Arlequin v3.5 software.Y-STR loci length polymorphism was compared against sequence polymorphism.Results A total of 106 haplotypes were observed in 108 unrelated males from Yunnan Miao-ethnic male individuals.The overall haplotype diversity(HD)and discrimination capacity(DC)were 0.9993 and 0.9815,respectively.Totally,204 genes were detected in 24 Y-STR loci,with their value of gene diversity(GD)ranging from 0.2177 to 0.9481.The GD values of 15 out of the 24 loci were over 0.6,and sequence polymorphism was observed in DYF387S1,DYS390,DYS389II,DYS437,DYS438,DYS448 and DYS612.Conclusion The 24 Y-STR loci showed high genetic polymorphism in the Yunnan Miao-ethnic male individuals,capable of providing reference for Y-STR database construction,population genetics and forensic practice.

关 键 词：法医物证学 二代测序 Y染色体短串联重复序列 序列多态性 

分 类 号：DF795.2[医药卫生—法医学]