pH和温度敏感的PTX-DEGMA接枝壳聚糖聚合物的制备及药物释放  

Preparation and Drug Release of PTX-Loaded DEGMA-Grafted Chitosan Polymer with pH and Temperature-Sensitivity

作　　者：张雪静 牛世伟 朱利民[1] ZHANG Xuejing;NIU Shiwei;ZHU Limin(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《东华大学学报（自然科学版）》2020年第2期232-236,共5页Journal of Donghua University(Natural Science)

基　　金：上海科委国际科技合作基金资助项目(16410723700);教育部生物医用纺织材料111引智专项资助项目(B07024)。

摘　　要：利用可逆加成断裂链转移(reversible addition-fragmentation chain transfer,RAFT)聚合法合成了二聚乙二醇单甲醚甲基丙烯酸甲酯(DEGMA)接枝的pH和温度敏感的壳聚糖(chitosan,CS)聚合物(CS-g-PDEGMA)。CS-g-PDEGMA与紫杉醇(paclitaxel,PTX)在水相中自组装形成CS-g-PDEGMA/PTX纳米颗粒。用核磁共振氢谱(1H-NMR)、紫外分光光度计、透射电子显微镜(TEM)以及动态光散射仪(DLS)对CS-g-PDEGMA聚合物进行表征。结果表明,该聚合物的低临界溶解温度(lower critical solution temperature,LCST)为33℃,呈球形且分散良好。体外释药研究表明,CS-g-PDEGMA聚合物有pH和温度响应性,且CS-g-PDEGMA/PTX纳米颗粒的药物缓释效果较好。由此可知该聚合物有望在生物医学领域中得到应用。The di(ethylene glycol)methyl ether methacrylate(DEGMA)grafted chitosan(CS)polymer(CS-g-PDEGMA)was synthesized through reversible addition-fragmentation chain transfer(RAFT)polymerization.The nano-particles of CS-g-PDEGMA/PTX were obtained by self-assemble method in the aqueous phase with CS-g-PDEGMA and paclitaxel(PTX).The polymer was characterized by nuclear magnetic resonance spectroscopy(1H-NMR),ultraviolet spectrophotometer,transmission electron microscopy(TEM)and dynamic light scattering(DLS).The results show that the lower critical solution temperature(LCST)of the polymer is 33℃,and the polymer is spherical and well dispersed.The in vitro drug release proves that the polymer is pH and temperature responsive and the nano-particles have a good sustained release effect.The above results indicate that the polymer is expected to be applied in the biomedical field.

关 键 词：壳聚糖 可逆加成断裂链转移聚合 二聚乙二醇单甲醚甲基丙烯酸甲酯 纳米载体 药物缓释 

分 类 号：O636.1[理学—高分子化学]