T细胞发育在系统性红斑狼疮发病机制中的作用  

Effects of T cell development in the pathogenesis of systemic lupus erythematosus

作　　者：陈金 陈旭艳 CHEN Jin;CHEN Xuyan(Department of Hematology and Rheumatology,the Second Affiliated Hospital of Xiamen Medical College,Xiamen 361101,China)

机构地区：[1]厦门医学院附属第二医院血液风湿科,福建厦门361101

出　　处：《中国现代医生》2020年第7期11-14,共4页China Modern Doctor

基　　金：福建省厦门市科技计划医疗卫生项目(3502Z20184062)。

摘　　要：目的探讨T细胞发育在系统性红斑狼疮(SLE)发病机制中的作用。方法选取2017年9月~2019年8月我院收治的活动期SLE患者120例为病例组,选取同期我院中青年健康体检者50例为对照组。检测CD4^+、CD8^+、CD4^+CD25^+和CD8^+CD25^+T细胞百分率,血清TGF-β、IL-10和IL-12水平,分析T细胞亚群分布特点及活化与SLE活动度评分、TGF-β、IL-10和IL-12水平的相关性。结果病例组的CD4^+、CD4^+CD25^+T细胞百分率与对照组比较,差异无统计学意义(P>0.05)。病例组的CD8^+T细胞百分率高于对照组(P<0.05),CD4^+/CD8^+比值低于对照组(P<0.05),CD8^+CD25^+T细胞百分率低于对照组(P<0.05),CD4^+CD25^+/CD8^+CD25^+比值高于对照组(P<0.05)。病例组的血清TGF-β水平低于对照组(P<0.05),IL-10和IL-12水平高于对照组(P<0.05)。CD4^+、CD8^+、CD4^+CD25^+和CD8^+CD25^+与SLE活动度评分、TGF-β水平无明显相关关系(P>0.05)。CD4^+CD25^+和CD8^+CD25^+与血清IL-10和IL-12水平呈负相关(P<0.05)。结论SLE患者存在T细胞发育中CD4^+、CD8^+亚群分布紊乱和活化异常,可能通过细胞因子TGF-β、IL-10和IL-12参与SLE的发病机制。Objective To investigate the effects of T cell development on the pathogenesis of systemic lupus erythematosus(SLE).Methods A total of 120 active-phase SLE patients admitted in our hospital from September 2017 to August 2019 were enrolled in the SLE group,and 50 healthy participants at young or middle age for physical examinations in the same period were enrolled in the control group.The proportions of CD4^+,CD8^+,CD4^+CD25^+and CD8^+CD25^+T cells and serum levels of transforming growth factorβ(TGF-β),interlukin-10(IL-10)and IL-12 were determined.Correlations between the distribution and activation of T-lymphocyte subsets and SLE disease activity index(SLEDAI),serum levels of TGF-β,IL-10 and IL-12 were analyzed.Results There were nonsignificant differences in the proportions of CD4^+T cells and CD4^+CD25^+T cells between the SLE group and the control group(P>0.05).The proportion of CD8^+T cells in the SLE group was higher than that in the control group(P<0.05).CD4^+/CD8^+ratio in the SLE group was lower than that in the control group(P<0.05).The proportion of CD8^+CD25^+T cells in the SLE group was lower than that in the control group(P<0.05).CD4^+CD25^+/CD8^+CD25^+ratio in the SLE group was higher than that in the control group(P<0.05).The serum level of TGF-βwas lower and levels of IL-10 and IL-12 were higher in the SLE group than those in the control group(P<0.05).There were nonsignificant correlations between CD4^+,CD8^+,CD4^+CD25^+and CD8^+CD25^+and SLEDAI,and between CD4^+,CD8^+,CD4^+CD25^+and CD8^+CD25^+and TGF-βlevels(P>0.05).CD4^+CD25^+and CD8^+CD25^+were negatively correlated with serum levels of IL-10 and IL-12(P<0.05).Conclusion The uneven distribution and abnormal activation of CD4^+and CD8^+T-lymphocyte subsets in T cell development in SLE patients can be involved in the pathogenesis of SLE via the regulation of cytokines,such as TGF-β,IL-10 and IL-12.

关 键 词：系统性红斑狼疮 T淋巴细胞 CD4+ CD8+ CD25+ 发病机制 

分 类 号：R593.2[医药卫生—内科学]