花(鱼骨)胚胎发育观察  

Observing the embryonic development of Hemibarbus maculatus Bleeker

作　　者：王静 方富贵 孟轲 乔刘鑫 孔浩强 郭晗 张益民 刘慧芬 李学军 Wang Jing;Fang Fugui;Meng Ke;Qiao Liuxin;Kong Haoqiang;Guo Han;Zhang Yimin;Liu Huifen;Li Xuejun(College of Fisheries,Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Henan Normal University,Xinxiang 453007,Henan China)

机构地区：[1]河南师范大学水产学院河南省水产动物养殖工程技术研究中心,河南新乡453007

出　　处：《河南水产》2019年第6期15-19,44,共6页HENAN FISHERIES

基　　金：河南省科技攻关重点项目(182102110383);河南师范大学青年科学基金项目;农业部热带亚热带水产资源利用与养殖重点实验室开放课题。

摘　　要：本实验利用蔡司倒置显微镜对花(鱼骨)胚胎发育过程进行观察研究.通过3种前处理方法比较,发现胚胎不做脱粘处理直接置于蒸馏水中效果最好.根据花(鱼骨)胚胎发育过程的形态变化,将其分为受精卵形成时期,卵裂时期,囊胚时期,原肠胚时期,神经胚期时期,器官形成期和出膜期.在水温24℃ 时,花(鱼骨)66 h孵化出膜.本文研究了花(鱼骨)胚胎发育的特点及过程,为生产性育苗工艺的建立具有重要意义.Using Zeiss inverted microscope,this experiment observed Hemibarbus maculatus Bleeker embryonic development process.By comparing the three pretreatment methods,it found that the effect of direct placement of embryos in distilled water without debonding treatment was the best.According to the morphological changes of its development process,it can be divided into seven stages:the formation of fertilized eggs,cleavage stage,blastocyst stage,gastrula stage,neuroembryonic stage,organogenesis stage and membranous stage.Membranes were hatched from Hemibarbus maculatus for 66 hours at water temperature of 24℃.This paper studied the characteristics and process of embryonic development of Hemibarbus maculatus,which is of great significance for the establishment of productive seedling raising technology.

关 键 词：花(鱼骨) 胚胎发育 观察 

分 类 号：S917.4[农业科学—水产科学]