CNQX拮抗断尾诱发小鼠前扣带回皮质神经元c-fos基因的表达  

Tail amputation induced c-fos gene expression in the anterior cingulate cortex neurons is antagonized by CNQX in mice

作　　者：李娜然[1] 商丽宏[1] 杨宇[2] 姚阳[2] 吴敏范[2] LI Naran;SHANG Lihong;YANG Yu;YAO Yang;WU Minfan(Center of Functional and Morphological Experiment,Shenyang Medical College,Shenyang 110034,China;Department of Physiology,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院机能与形态实验中心,沈阳110034 [2]沈阳医学院生理学教研室,沈阳110034

出　　处：《中国医科大学学报》2020年第4期318-320,共3页Journal of China Medical University

基　　金：辽宁省科学技术计划(L2015020391);沈阳医学院科学研究基金(20151009)。

摘　　要：目的研究谷氨酸AMPA/Kainate受体拮抗剂CNQX对断尾诱发小鼠前扣带回皮质神经元c-fos基因表达的影响。方法免疫组化方法分别研究尾静脉注射和蛛网膜下腔注射CNQX对尾末端2.5 cm剪断后0.5 h小鼠前扣带回皮质神经元c-fos基因表达的影响。结果正常小鼠前扣带回皮质神经元有少量c-fos基因表达,而断尾后0.5 h小鼠前扣带回皮质神经元c-fos基因表达显著增强。同时,尾静脉注射和蛛网膜下腔注射CNQX均能拮抗该c-fos基因表达的显著增强。结论断尾后小鼠前扣带回皮质神经元c-fos基因表达显著增强的过程有外周与中枢谷氨酸AMPA/Kainate受体参与。Objective We aimed to study the effect of glutamate AMPA/kainate receptor antagonist CNQX on tail amputation induced c-fos gene expression in the anterior cingulate cortex neurons of mice.Methods CNQX was injected into the caudal vein or subarachnoid space.The effect of CNQX on gene expression at 0.5 h after cutting 2.5 cm of the tail extremity was studied using immunohistochemistry.Results In the control group,a small amount of c-fos gene was expressed in the anterior cingulate cortex neurons.However,the expression of c-fos in the anterior cingulate cortex neurons of mice increased significantly 0.5 h after tail amputation;this increase was antagonized by CNQX injection into the caudal vein or subarachnoid space.Conclusion Glutamate peripheral and central AMPA/kainate receptors are involved in tail amputation induced increase in c-fos gene expression in the anterior cingulate cortex neurons of mice.

关 键 词：幻肢痛 前扣带回皮质 AMPA/Kainate受体 C-FOS基因 

分 类 号：R338[医药卫生—人体生理学] Q432[医药卫生—基础医学]