重金属Cu^2+胁迫对绿豆生理生化指标的影响  

Effects of Heavy Metal Cu^2+Stress on Physiological-biochemical Characteristic of Mung Bean

作　　者：庄枫红 张晓娟[1] ZHUANG Feng-hong;ZHANG Xiao-juan(School of Life Sciences,South China Normal University,Guangzhou,Guangdong 510631)

机构地区：[1]华南师范大学生命科学学院,广东广州510631

出　　处：《安徽农业科学》2020年第8期43-46,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探究重金属Cu^2+对绿豆幼苗生长发育的影响,以期为进一步研究重金属Cu^2+对植物生长发育的毒害机制提供参考。[方法]绿豆在水培(1/2 Hoaglands营养液)条件下,外源添加0,100,200,400,600 mg/L的CuSO 4进行胁迫处理,测定其对绿豆幼苗株高和鲜重,叶片叶绿素含量、丙二醛含量、可溶性糖含量以及抗氧化酶活性的影响。[结果]随着Cu^2+浓度的增加,绿豆幼苗的平均株高和平均鲜重受抑制程度逐渐升高,其中最高抑制率分别为45.4%和53.8%;Cu^2+胁迫下的绿豆叶片叶绿素含量均高于对照组,呈先上升后下降的趋势;丙二醛及可溶性糖含量随Cu^2+浓度增加而升高,最高增长率分别为132%和80.9%;绿豆叶片受到Cu^2+胁迫后,其抗氧化酶活性均有不同程度的升高,SOD、POD及CAT活性最高增长分别为对照的2.36,2.29和2.53倍。[结论]绿豆幼苗在受到重金属Cu^2+胁迫时,其生长被显著地抑制,叶片细胞膜系统受到损伤,但其叶绿素、可溶性糖含量及抗氧化酶活性升高,表现出一定的抗性。[Objective]Mung bean was used as material to explore the effects of heavy metal Cu^2+on its growth and development,so as to provide reference for further studying of the toxic mechanism of heavy metal Cu^2+on plant growth and development.[Method]Under the condition of water culture(1/2 Hoaglands nutrient solution),different concentrations of CuSO 4(0,100,200,400,600 mg/L)were added for stress treatment,and the effects of CuSO 4 on the plant height and fresh weight of mung bean,the content of chlorophyll,malondialdehyde and soluble sugar and the antioxidant enzyme activity of leaves were measured.[Result]With the increase of Cu^2+concentration,the inhibition degree of the average plant height and fresh weight of mung bean increased gradually,and the highest inhibition rate was 45.4%and 53.8%respectively.The chlorophyll content of mung bean leaves in the stress treatment group was higher than that in the control group,which showed a trend of increasing at first and decreasing later.The content of malondialdehyde and soluble sugar increased with the increase of Cu^2+concentration,with the highest growth rates of 132%and 80.9%respectively.Besides,the antioxidant enzyme activity of mung bean leaves increased when treated with Cu^2+.The highest activity of SOD,POD and CAT rose 2.36,2.29 and 2.53 times,respectively.[Conclusion]Under the stress of heavy metal Cu^2+,the growth of mung bean was significantly inhibited and the cell membrane system was damaged,but the contents of chlorophyll,soluble sugar and antioxidant enzyme activity increased,showing certain resistance.

关 键 词：绿豆 CU 2+胁迫 生长发育 生理生化指标 

分 类 号：Q945.78[生物学—植物学] H3[语言文字]