超声-酶提取毛麝香工艺优化及抗氧化活性研究  

Study on Ultrasonic-enzyme-assisted Extraction Process and Antioxidant Activity of Adenosma glutinosum

作　　者：王呈文[1] 蔡映举 范佳伟 林少弟 WANG Cheng-wen;CAI Ying-ju;FAN Jia-wei(Shantou Polytechnic,Shantou,Guangdong 515078)

机构地区：[1]汕头职业技术学院,广东汕头515078

出　　处：《安徽农业科学》2020年第8期172-174,188,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化毛麝香全草的超声-酶辅助法提取工艺,并评价其抗氧化活性。[方法]采用正交试验优选最佳工艺。运用DPPH法、ABTS法、NADH-PMS-NBT反应体系法和FRAP法测定总抗氧化能力4种体外抗氧化活性检测方法,并以V C为阳性对照,对比研究毛麝香4种不同提取物的抗氧化活性。[结果]最优工艺条件为乙醇浓度75%、料液比1∶40(g∶mL)、超声温度60℃、超声时间30 min、纤维素酶用量0.6%,此条件下的提取率为3.48%。毛麝香乙酸乙酯相的抗氧化能力最佳,对清除3种自由基(DPPH·、ABTS+·、O 2·-)的IC 50分别为126、156和512μL,总抗氧化能力测定值为16.7 mg/g。[结论]该提取方法简便、可行,提取物具有良好的抗氧化能力。[Objective]The research aimed to optimize the ultrasonic-enzyme-assisted extraction process of Adenosma glutinosum and evaluate its antioxidant activity.[Method]The best process was preferred using orthogonal test.Four methods of in vitro antioxidant activity detection by DPPH method,ABTS method,NADH-PMS-NBT reaction system method and FRAP method were used,and V C was used as a positive control.The antioxidant activities of four different extracts of Adenosma glutinosum were compared.[Result]The optimal process conditions were obtained as follow:ethanol concentration of 75%,solid-liquid ratio of 1∶40(g∶mL),ultrasonic temperature of 60℃,ultrasonic time of 30 min and cellulase dosage of 0.6%.The extraction rate of this condition was 3.48%.The ethyl acetate fraction of Adenosma glutinosum had the strongest antioxidant capacity.The IC 50 of ethyl acetate fraction for removing three kinds of free radicals(DPPH·,ABTS+·,O 2·-)was 126,156 and 512μL,respectively,and the total antioxidant capacity was 16.7 mg/g.[Conclusion]The extraction method is simple and feasible,and the extract exhibited strong antioxidant activity.

关 键 词：毛麝香 超声-酶辅助 工艺优化 抗氧化 

分 类 号：R284[医药卫生—中药学]