正交试验优选龙胆炮制工艺的研究  

Study on Optimization of the Processing Technology of Gentiana by Orthogonal Experiment

作　　者：廖梅香[1] 薛玉洁 LIAO Mei-xiang;XUE Yu-jie(School of Pharmacy,Gannan Medical College,Ganzhou,Jiangxi 341000;Grade 2015 of Chinese Pharmacy Specialty,Gannan Medical College,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院药学院,江西赣州341000 [2]赣南医学院2015级中药学专业,江西赣州341000

出　　处：《安徽农业科学》2020年第8期182-184,共3页Journal of Anhui Agricultural Sciences

基　　金：江西省中医药课题(2016A054);江西省教育厅课题(GTT180817)。

摘　　要：[目的]比较龙胆生品、酒炙、姜炙、甘草炙、炒炭等不同炮制品中龙胆苦苷的含量,并利用正交试验进一步优化其炮制工艺,探讨提高龙胆苦苷含量的最佳炮制工艺。[方法]分别对龙胆药材进行不同的炮制处理,采用超声法进行提取,利用高效液相色谱法(HPLC)分别测定其含量,选择龙胆苦苷含量最高的样品进行正交试验,进一步优化炮制工艺。[结果]龙胆不同炮制品中酒炙龙胆的含量高于其他炮制品。当炮制温度为100℃,闷润时间为1 h,龙胆与黄酒用量比例为5∶1时,龙胆苦苷含量最高。[结论]酒炙法是适用于龙胆的炮制方法,且最适宜的炮制条件为炮制温度100℃、闷润时间1 h、龙胆与黄酒用量比例5∶1。[Objective]To compare the content of gentiopicroside from Gentiana scabra Bge.in different processing technology such as the crude,carbonized products,and stir-frying with wine,ginger,glycyrrhiza products,further discuss and analyze the best processing method for increasing the content of gentiopicroside.[Method]HPLC method was applied to determine the content of gentiopicroside with ultrasonic extraction from different processed products.By comparison and analysis,the orthogonal test was carried out on the product with the highest content to optimize processing technology.[Result]In different processing products,the content of gentiopicroside in stir-frying with wine products was higher than other products,and the content was highest when the temperature was 100℃,the moist time was 1 h,the proportion of G.scabra Bge.and wine was 5∶1.[Conclusion]The optimum method to process G.scabra Bge.was stir-frying with wine,and the concrete conditions were as follows:temperature was 100℃,the moist time was 1 h,the proportion of G.scabra Bge.and wine was 5∶1.

关 键 词：龙胆苦苷 高效液相色谱法 超声提取法 正交试验 

分 类 号：R283[医药卫生—中药学]