白藜芦醇对小细胞肺癌C-myc基因表达的调控研究  

Study of regulating effect of resveratoral on C-myc gene in human small cell lung cancer H446 cells

作　　者：李王平 马李杰 潘蕾 金发光 Li Wangping;Ma Lijie;Pan Lei;Jin Faguang(Department of pulmonary and critical care medicaine, Tangdu Hospital, The Air Force Medical university, Xi′an 710038, China)

机构地区：[1]空军(第四)军医大学第二附属医院呼吸与危重症医学科,西安710038

出　　处：《中华肺部疾病杂志（电子版）》2020年第2期148-153,共6页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：国家自然科学基金资助项目(81071933)。

摘　　要：目的研究白藜芦醇对小细胞肺癌C-myc基因表达的影响,探讨其在小细胞肺癌中的可能抑癌机制。方法以人小细胞肺癌H446细胞系为研究对象,采取Western blot法和Real-Time PCR法检测不同浓度白藜芦醇及白藜芦醇作用不同时间后C-myc的表达变化,分别以NAC和顺铂为干预进行验证。结果Western blot和Real-time PCR检测结果显示白藜芦醇可调控人小细胞肺癌H446细胞C-myc的表达,并随着作用时间的延长和作用浓度的增加,C-myc的表达进一步下降,具有时间依赖和浓度依赖的特点,差异有统计学意义(P<0.05)。给予NAC干预后,可抑制白藜芦醇对C-myc蛋白表达的调控影响,呈现拮抗作用,差异均有统计学意义(P<0.05)。顺铂可抑制C-myc的表达,与白藜芦醇联用对C-myc调控具有协同作用,Western blot检测结果显示在胞浆蛋白中差异有统计学意义(P<0.05),但在胞核蛋白中,与白藜芦醇组相比,白藜芦醇与顺铂联用,差异无统计学意义(P>0.05)。Real-time PCR检测结果显示顺铂可使C-myc表达水平下调,与白藜芦醇联用具有协同作用,差异有统计学意义(P<0.05)。结论白藜芦醇对小细胞肺癌H446细胞的C-myc表达具有明显的影响,间接说明白藜芦醇对小细胞肺癌中的抑癌作用可能与myc基因有关。Objective The aim of the present study was to evaluate the effects of resveratrol on C-myc gene expression in small-cell lung cancer(SCLC)cell.Methods The H446 cell line of human small-cell lung cancer was used as the object of this study.The expression levels of C-myc were measured by western blot analysis and Real-time PCR in H446 cells treated with various concentrations of Res for 24 h and treated with the same concentration Res at different times.Cisplatin was used as the intervention for effect verification.Results The measure results of Western blot and Real-time PCR show that resveratrol has regulating effect on the expression of C-myc in H446 cells of human small-cell lung cancer.Along with the extension of the action time and the increase of the concentration,the expression of c-myc further decreased.These effects have the characteristic of concentration-and time-dependence,the difference has statistically significant(P<0.05).In addition,combined treatment with NAC and Res is found to be a inhibiting effect compared with either monotherapy while cisplatin and Res is found to be markedly more effective compared with either monotherapy,the difference has statistically significant(P<0.05).However,the result of Western blot shows that the expression of C-myc in the cytonuclear protein has no significant difference in the combination group and the resveratrol group(P>0.05).Conclusion The present study demonstrated that resveratrol has a significant effect on C-myc expression in H446 cells.These findings suggest that the inhibiting effect of resveratrol on small cell lung cancer may be related to myc gene.

关 键 词：白藜芦醇 小细胞肺癌 H446细胞 抑癌基因 

分 类 号：R563[医药卫生—呼吸系统]