番茄长花柱性状遗传规律与QTL分析  

Inheritance and QTL Analysis of Long-style Trait in Tomato

作　　者：马雅琳 梁燕[1] MA Yalin;LIANG Yan(College of Horticulture,Northwest A&F University,Yangling,Shaannxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2020年第2期264-274,共11页Acta Horticulturae Sinica

基　　金：国家“十三五”重点研发计划项目(2016YFD0101703)。

摘　　要：以长花柱番茄‘J59’为材料对其长花柱性状的温度敏感性、遗传规律以及相关QTL进行分析:对两叶一心幼苗分别进行昼夜18/10℃、25/18℃和35/25℃处理,发现雌蕊长度、雄蕊长度、花柱长度和柱头外露长度无显著差异,表明‘J59’番茄不是温度敏感型长花柱材料;以长花柱番茄‘J59’和短花柱番茄‘M82’为亲本,构建了6个世代遗传分析群体P1、P2、F1、BC1P1、BC1P2和F2,结果显示长花柱性状遗传规律符合MX2-ADI-AD模型,以主基因遗传为主,BC1P1、BC1P2和F2群体的主基因遗传率分别为65.51%、90.76%和89.10%;以F2世代为分析群体构建了遗传连锁图谱,其包含121个SSR,12个连锁群,覆盖基因组长度2108.71cM,平均标记间距为17.43cM;共检测到10个与长花柱性状相关的QTL,分布于Chr1、Chr2、Chr3、Chr4、Chr5、和Chr12,LOD值介于2.53~26.85之间,可解释1.28%~20.22%的表型变异率,其中高于10%的位点有3个,占QTL总数的30%。In this study,the temperature sensitivity,inheritance and QTL(quantitative trait locus)analysis of long-style trait were performed using a tomato(Solanum lycopersicum)material,J59,which shows a long style phenotype.The main results were as follows:when the seedlings at two-leaf stage were treated with different temperature conditions,including 18/10℃,25/18℃,and 35/25℃in day/night,respectively,the lengths of pistil,stamen,style,and stigma exsrtion were not significantly changed,implying that the tomato J59 is not a temperature-sensitive material.Furthermore,the six generations of genetic groups,namely,P1,P2,F1,BC1P1,BC1P2,and F2,were constructed using the J59 and M82(short style)tomatoes as parental materials.Genetic analysis indicated that the long style trait conforms to the MX2-ADI-AD model and is mainly controlled by major genes.The inheritabilities of major genes in BC1P1,BC1P2 and F2 populations are 65.51%,90.76%and 89.10%,respectively.Moreover,using 121 polymorphic SSR markers,a genetic linkage map was constructed,which consists of 12 linkage groups,and covers a total length of 2108.71 cM in the genome with an average of 17.43 cM between the adjacent markers.The QTL analysis was performed based on the inclusive composite interval mapping method.Ten QTLs associated with long-style trait,which distribute in Chr1,Chr2,Chr3,Chr4,Chr5,and Chr12,were detected.LOD scores are ranged from 2.53 to 26.85.These QTLs can explain the phenotypic variability of 1.28%–20.22%,in which,the variabilities of 3 QTLs are higher than 10%,accounting for 30%of the total QTLs.

关 键 词：番茄 长花柱 遗传模型 遗传率 QTL定位 

分 类 号：S641.2[农业科学—蔬菜学]