基于频率调制和空间编码的单PMT荧光分子断层成像  

Single PMT Fluorescence Molecular Tomography Based on Frequency Modulation and Spatial Coding

作　　者：陈若仲 王琳[1] 陈雪利[2] 侯榆青[1] 贺小伟[1] Chen Ruozhong;Wang Lin;Chen Xueli;Hou Yuqing;He Xiaowei(School of Information Technology,Northwest University,Xi′an,Shaanxi 710127,China;School of Life Science,Xidian University,Xi′an,Shaanxi 710126,China)

机构地区：[1]西北大学信息科学与技术学院,陕西西安710127 [2]西安电子科技大学生命科学学院,陕西西安710126

出　　处：《中国激光》2020年第1期232-241,共10页Chinese Journal of Lasers

基　　金：国家自然科学基金(11571012,61601363);陕西省教育厅服务地方专项计划(17JF027)。

摘　　要：针对荧光分子断层成像数据采集方式存在的问题,提出了一种基于频率调制和空间编码的成像方法,旨在改进数据采集方案,缩短数据采集时间。在该方法中,激发光束被分成若干个子束,用作多点激发光源。这些子光束首先被调制成不同的频率,然后同时入射到目标表面的不同点上。在检测端,目标的出射光首先通过空间编码掩模,然后被引导至单光电倍增管。根据压缩感知理论,改变掩模的模式,进行稀疏重构恢复,最终得到目标表面荧光信号的分布。为了验证本文所提方法的可行性,设计了相应的仿真模拟实验,实验结果表明该方法可以较好地恢复原始图像,证明该方法的可行性。Herein,we propose an imaging method based on frequency modulation and spatial coding to overcome the problem associated with fluorescence molecular tomography data acquisition methods,improve the data acquisition scheme,and reduce data acquisition time.In this method,the excitation beam is split into several sub-beams and used as multipoint excitation source.These sub-beams are modulated to different frequencies and then simultaneously incident at different points on the target surface.At the detection side,the emergent light of the target is first directed to a single photomultiplier through a spatially coded mask.According to the compressed sensing theory,the distribution of fluorescence signals on the target surface can be obtained by changing the mask mode and conducting sparse reconstruction and recovery.We design the corresponding simulation experiment to evidence the proposed methodology.Result shows that the method can better restore the original image,which proves the feasibility of the proposed method.

关 键 词：医用光学 荧光分子断层成像 频率调制 空间编码 压缩感知理论 光电倍增管 

分 类 号：TP391[自动化与计算机技术—计算机应用技术] Q632[自动化与计算机技术—计算机科学与技术]