黄淮麦区小麦新品种(系)抗条锈水平与抗病基因分析  

Stripe Rust Resistance of New Varieties(Lines)from Huang-Huai Valley Wheat Region in China

作　　者：蔚睿 金彦刚 吴舒舒 吴建辉 王琪琳[1,4] 曾庆东 刘胜杰[1,2] 夏中华 王晓静[1] 康振生 韩德俊 YU Rui;JIN Yangang;WU Shushu;WU Jianhui;WANG Qilin;ZENG Qingdong;LIU Shengjie;XIA Zhonghua;WANG Xiaojing;KANG Zhensheng;HAN Dejun(State Key Laboratory of Crop Stress Biology in Arid Areas,Northwest A&F University,Yangling,Shaanxi 712100,China;College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;Jiangsu Ruihua Agricultural Science &Technology Co,Ltd,Suqian,Jiangsu 223800,China;College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100 [3]江苏瑞华农业科技有限公司,江苏宿迁223800 [4]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《麦类作物学报》2020年第3期278-284,共7页Journal of Triticeae Crops

基　　金：国家重点研发计划项目(2018YFD0200405);江苏省科技成果转化专项(BA2017138);国家重点研发计划项目(2017YFD0100701);国家自然科学基金项目(31971890)。

摘　　要：了解黄淮麦区近几年小麦育成品种(系)对条锈菌主要流行小种的抗性表现及其抗条锈病基因的分布状况,利用当前流行的条锈菌小种条中32、条中33和条中34,对近年参加审定的150份小麦品种(系)进行苗期分小种鉴定,同时分别在杨凌设置人工接种病圃,在天水和江油设置自然诱发病圃,进行成株期抗条锈性鉴定,并结合Yr5、Yr7、Yr9、Yr10、Yr17、Yr18、Yr26和YrSP等8个已知抗条锈病基因的分子标记进行分子检测。结果表明,在150份材料中,47份表现为成株期抗性(占31.3%),31份表现为慢锈性(占20.7%),未发现具有全生育期抗锈病材料,其余材料均表现感病;11份材料检测含有Yr7(占7.3%),104份检测含有Yr9(占69.3%);13份检测含有Yr17(占8.7%);10份同时检测到含有Yr9和Yr17(占6.7%);3份同时检测到含有Yr9和Yr18(占2%);2份同时检测到含有Yr9、Yr17和Yr18(占1.3%),含有Yr7和Yr17,Yr7和Yr9,Yr9和YrSP的材料各1份(占0.7%),未检测到含有Yr5、Yr10和Yr26的材料,可能个别品种携带抗条锈病新基因。以上结果说明,黄淮麦区小麦品种(系)综合抗性较10年前已获得大幅度提高,其抗性主要来自于基因组合,如Yr17、Yr18与其他基因组合会增强小麦抗性。部分具有良好抗性的材料的抗性基因有待进一步遗传解析。Accurate evaluation of wheat resistance to the predominant Puccinia striiformis f.sp.tritici(Pst)races and understanding the corresponding components of resistance genes in wheat germplasm would provide insight into rational use and layout of new varieties.For this purpose,a total of 150 wheat varieties(lines)from Huang-Huai Valley Wheat Region were evaluated for their all-stage resistance(ASR)with predominant races CYR32,CYR33 and CYR34 under a control greenhouse and adult plant resistance(APR)was assessed in field under artificial inoculation and natural infection environments.In addition,these materials were also detected using various molecular markers linked with Yr genes including Yr5,Yr7,Yr9,Yr10,Yr17,Yr18,Yr26 and YrSP.Among the 150 tested varieties(lines),no material had ASR,but 47 materials displayed APR(accounting for 31.3%),and 31 materials showed slow rusting(accounting for 20.7%)and the remainings were susceptible.Resistance genes Yr7,Yr9 and Yr17 were postulated in 11,104 and 13 materials,respectively.Yr gene combinations Yr7+Yr9,Yr7+Yr17,Yr9+Yr17,Yr9+Yr18,Yr9+YrSP,Yr9+Yr17+Yr18 were also postulated in 1,1,10,3,1,2 materials,respectively.However,no material carried Yr5,Yr10 and Yr26.In addition,individual cultivars may carry new genes resistant to stripe rust but further genetic mapping analysis should be performed for confirmation.The above results showed that the comprehensive resistance of wheat varieties(lines)in Huang-Huai Valley wheat region was significantly improved compared with that 10 years ago,and the resistance mainly derived from gene combinations,such as Yr17,Yr18 and other gene combinations,which could enhance the resistance of wheat.The resistant genes of some materials with good resistance need further genetic analysis.

关 键 词：黄淮麦区 小麦条锈病 小麦参试材料 抗病基因 标记辅助检测 

分 类 号：S512.1[农业科学—作物学] S330