透钙磷石骨水泥复合明胶及重组人骨形态发生蛋白2/7修复骨缺损  

Calcium hydrogen phosphate dehydrate combined with gelatin and recombinant human bone morphologic protein 2/7 for repair of bone defects in rabbits

作　　者：李雪微 胡蓓蓓 张大伟 全璐璐 梁永强 Li Xuewei;Hu Beibei;Zhang Dawei;Quan Lulu;Liang Yongqiang(Department of Orthodontics,College of Stomatology,North China University of Science and Tech no logy,Tangshan 063000,Hebei Province,China;Department of Stomatology,Ankang Hospital of Traditional Chinese Medicine,Xi'an 725000,Shaanxi Province,China)

机构地区：[1]华北理工大学口腔医学院正畸科,河北省唐山市063000 [2]安康市中医医院口腔科,陕西省西安市725000

出　　处：《中国组织工程研究》2020年第28期4533-4539,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81270965),项目参与者:梁永强;河北省教育厅重点项目(ZD2015016),项目负责人:梁永强.

摘　　要：背景:课题组前期研究发现10%锶-透钙磷石的成骨能力高于透钙磷石和5%锶-透钙磷石,但也发现掺锶透钙磷石的孔结构不够理想,前期成骨效果不佳。目的:在10%锶-透钙磷石中加入明胶和重组人骨形态发生蛋白2/7(recombinant Human bone morphogenetic proteins 2/7,rh BMP2/7),观察其修复家兔下颌骨缺损的成骨效果。方法:分别制备明胶-10%锶-透钙磷石与含0.04,1 g/L rh BMP2/7的明胶-10%锶-透钙磷石材料。在45只家兔双侧下颌骨制作骨缺损模型,分5组干预:空白对照组不植入任何材料,其余4组分别植入10%锶-透钙磷石(对照组)、明胶-10%锶-透钙磷石(明胶组)、0.04g/Lrh BMP2/7-明胶-10%锶-透钙磷石(0.04g/L rhBMP2/7组)、1 g/L rhBMP2/7-明胶-10%锶-透钙磷石(1 g/L rhBMP2/7组)。术后4,8,12周取骨缺损标本,分别进行锥形束CT与免疫组化观察。实验经华北理工大学实验动物伦理委员会审议批准。结果与结论:①锥形束CT:术后8周时,1g/Lrh BMP2/7组骨修复基本完成,新生骨组织与周围组织几乎融合;0.04g/Lrh BMP2/7组和胶原组缺损区大部分修复,新骨修复边缘不平整;对照组部分修复。术后12周时,明胶组、0.04,1 g/L rhBMP2/7组完成缺损区骨修复;②免疫组化观察:术后4,8周时,1 g/L rhBMP2/7组Ⅰ型胶原表达高于其他4组(P<0.05),0.04 g/L rhBMP2/7组、胶原组高于对照组(P<0.05);术后12周时,植入材料4组间Ⅰ型胶原表达比较差异无显著性意义(P>0.05);③结果表明:在10%锶-透钙磷石中加入明胶和1 g/L的重组人骨形态发生蛋白2/7可促进骨缺损的修复。BACKGROUND: Studies have shown that the osteogenic ability of 10% strontium-doped calcium hydrogen phosphate dihydrate is higher than that of calcium hydrogen phosphate dihydrate and 5% strontium-doped calcium hydrogen phosphate dihydrate, but also found that the pore structure of 10% strontium-doped calcium hydrogenphosphate dihydrate is not ideal, and the early osteogenic effect is not satisfactory. OBJECTIVE: To investigate the osteogenic effect of the composite of 10% strontium-doped calcium hydrogenphosphate dehydrate and gelatin and recombinant human bone morphogenetic protein 2/7(rhBMP2/7). METHODS: Gelatin-10% strontium-doped calcium hydrogenphosphate dihydrate and gelatin-10% strontium-doped calcium hydrogenphosphate dihydrate material containing 0.04 g/L and 1 g/L rhBMP2/7 were prepared respectively. Forty-five rabbit models of bilateral mandibular defects were prepared and then divided into five groups. In the blank control group, no material was implanted. 10% strontium-doped calcium hydrogen phosphate dihydrate(control group) and gelatin-10% strontium-doped calcium hydrogen phosphate dihydrate(gelatin group), 0.04 g/L rhBMP2/7-gelatin-10% strontium-doped calcium hydrogenphosphate dihydrate(0.04 g/L rhBMP2/7 group), and 1 g/L rhBMP2/7-gelatin-10% strontium-doped calcium hydrogenphosphate dihydrate(1 g/L rhBMP2/7 group) were implanted in the remaining four groups, respectively. Bone defect specimens were taken at 4, 8 and 12 weeks after surgery, and were examined by cone beam CT and immunohistochemistry. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China. RESULTS AND CONCLUSION: Cone beam CT examination revealed that at 8 weeks after surgery, bone repair was basically completed and the new bone tissue was almost fused with the surrounding tissue in the 1 g/L rhBMP2/7 group. Most of defect area was repaired, and the edge of new bone was unsmooth in the 0.04 g/L rhBMP2/7 and gelatin groups. Bone defect in the control group partially repaired.

关 键 词：透钙磷石 骨缺损 明胶 rhBMP2/7 Ⅰ型胶原 锶 骨修复 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]