响应曲面法优化贯叶连翘脂溶性成分的超声辅助提取工艺  

Optimization in Ultrasonic-Assisted Extraction Process of Fat-Soluble Components from Hypericum perforatum L.by Response Surface Methodology

作　　者：王燕[1] 高洁[1] 周蕊[1] WANG Yan;GAO Jie;ZHOU Rui(Shaanxi Institute of International Trade & Commerce,Xianyang 712046,China)

机构地区：[1]陕西国际商贸学院,陕西咸阳712046

出　　处：《化学与生物工程》2020年第4期34-38,共5页Chemistry & Bioengineering

基　　金：陕西省教育厅专项科研计划项目(18JK0950)。

摘　　要：以脂溶性成分提取率为指标,在单因素实验的基础上,采用响应曲面法优化贯叶连翘脂溶性成分的超声辅助提取工艺。确定最优提取工艺条件为:以正己烷为提取溶剂、超声功率200 W、提取温度48℃、提取时间41 min、液固比9∶1(mL∶g),在此条件下,贯叶连翘脂溶性成分提取率达到9.45%(相对标准偏差小于1%)。该提取工艺具有设备简单、耗时少、成本低等特点,为贯叶连翘有效成分的进一步研究提供了实验数据。Using the extraction rate of fat-soluble components as an index,we optimized the ultrasonic-assisted extraction process of fat-soluble components from Hypericum perforatum L.by response surface methodologies on the basis of single-factor experiments.The optimum extraction conditions are determined as follows:using n-hexane as the extraction solvent,the ultrasonic power of 200 W,the extraction temperature of 48℃,the extraction time of 41 min,and the liquid-solid ratio of 9∶1(mL∶g).Under above conditions,the extraction rate of fat-soluble components from Hypericum perforatum L.reaches 9.45%,and the RSD is less than 1%.This extraction process has the characteristics of simple equipment,time saving,and low cost,which provides experimental data for further research on the effective components from Hypericum perforatum L..

关 键 词：贯叶连翘 脂溶性成分 超声辅助提取法 响应曲面法 

分 类 号：R284.2[医药卫生—中药学]