肺结核患者血清差异表达环状RNA的筛选  

Screening analysis of differentially expressed circRNA in serum of patients with pulmonary tuberculosis

作　　者：张迪 赵辉[1] 王家路[1] ZHANG Di;ZHAO Hui;WANG Jialu(Center for Medical Laboratory, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China)

机构地区：[1]新疆医科大学第一附属医院医学检验中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2020年第4期434-438,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金(2017D01C314)。

摘　　要：目的探讨结核病患者血清差异表达环状RNA(circRNA)的筛选。方法收集肺结核患者(病例组)及健康对照者(对照组)各3例,利用表达谱芯片将病例组和对照组的全血circRNA进行检测并筛选差异表达的circRNA,将circRNA的差异基因通过基因本体(GO)富集分析和信号通路的显著性分析筛选出结核病差异基因。结果本研究发现3000个上调circRNA,2484个下调circRNA。具备差异特性的circRNA 835个(P<0.05),其中上调的circRNA 249个,下调的circRNA 586个。GO富集分析表明差异基因主要富集在突触间隙中乙酰胆碱分解代谢过程、对水浸抑制应力酸受体信号通路、受体循环调节、细胞外基质分解等生物学过程。信号通路的显著性分析表明差异基因主要参与阿尔茨海默氏病、子宫内膜癌、甲状腺癌、结核、噬菌体、系统性红斑狼疮、FcγR介导的吞噬作用等信号通路调节。结论结核病患者血清中存在差异表达的circRNA,差异circRNA通过其靶基因参与多个生物学过程和信号通路调节。Objective To explore the screening of circRNA in serum of tuberculosis patients.MethodsThree cases of tuberculosis patients and three healthy controls were collected,and the circRNAs of whole blood in the case group and the control group were detected.And the differential expression of circRNAs were screened using the expression profile chip.The differential genes of circRNA were screened for the differential genes of tuberculosis by means of gene ontology database enrichment analysis(GO Enrichment Analysis)and signal pathway significance analysis(pathway enrichment analysis).ResultsThis study found that 3000 up-regulated circRNA and 2484 down-regulated circRNA.There were 835 circRNAs with different characteristics(P<0.05),of which 249 were up-regulated and 586 were down-regulated.The enrichment analysis of the gene ontology database showed that the differential genes were mainly enriched in biological processes such as acetylcholine catabolism in the synaptic cleft,inhibition of the stress acid receptor signaling pathway by water immersion,receptor cycle regulation,and extracellular matrix breakdown.Significant analysis of signaling pathways indicates that differential genes were mainly involved in signal pathway regulation such as Alzheimer′s disease,endometrial cancer,thyroid cancer,tuberculosis,phage,systemic lupus erythematosus,and FcγR-mediated phagocytosis.ConclusionThere are differential expression of circRNAs in the serum of tuberculosis patients.Differential circRNAs are involved in the regulation of multiple biological processes and signaling pathways through their target genes.

关 键 词：结核病 信号通路 差异基因 

分 类 号：R855.2[医药卫生—航空、航天与航海医学]