腹部推拿对结肠慢传输型便秘大鼠的治疗效果  

Effect of abdominal massage on 5-HT and VIP in rats with slow transit constipation

作　　者：徐境阳 马鑫文 王新军[1,3] XU Jingyang;MA Xinwen;WANG Xinjun(College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China;Research Institute of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region;Department of Massage, the Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830002, China)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830011 [2]新疆维吾尔自治区中医药研究院 [3]新疆医科大学附属中医医院推拿科,乌鲁木齐830002

出　　处：《新疆医科大学学报》2020年第4期499-502,共4页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区科技厅研究项目(2017D01C174)。

摘　　要：目的研究腹部推拿对结肠慢传输型便秘(Slow Transit Constipation,STC)大鼠的治疗效果。方法取52只8周龄SD雌性大鼠用0.125%的苯扎氯铵溶液建立慢传输型便秘大鼠模型,建模后随机分为4组:模型对照组(蒸馏水1 mL/kg体质量)、药物组(西沙比利3 mg/kg体质量)、推拿组(腹部推拿)、药物+推拿组(西沙比利3 mg/kg体质量+腹部推拿),每组13只,另取13只正常大鼠为空白组(蒸馏水1 mL/kg体质量)。饲养10 d,收集各组大鼠造模前1天,造模第10天,干预第7天和干预第14天24 h的排便量,计算干湿比,记录大鼠的应激排便时间。采用酶联免疫法检测血清中五羟色胺(5-HT)、血管活性肠肽(VIP)含量。结果与空白组比较,其他各组大鼠造模第10天24 h排便量均降低,应激排便时间延长,排便干湿比升高,差异有统计学意义(P<0.05);模型组血清中5-HT含量降低,VIP含量升高,差异有统计学意义(P<0.05)。与模型组比较,药物组、推拿组、药物+推拿组大鼠干预第14天24 h排便量均升高,应激排便时间均缩短,排便干湿比均降低,血清中5-HT含量均升高,VIP含量均降低,差异有统计学意义(P<0.05)。与药物组比较,药物+推拿组大鼠干预第14天血清中VIP含量降低,差异有统计学意义(P<0.05)。与推拿组比较,药物+推拿组大鼠干预第14天血清中5-HT含量升高,VIP含量降低,差异有统计学意义(P<0.05)。结论腹部推拿对STC大鼠具有较好的治疗效果。Objective To study the therapeutic effect of abdominal massage on slow transit constipation rats.MethodsA total of 528-week-old SD female rats were used to establish slow transit constipation model with 0.125%benzalkonium chloride solution,after modeling,they were randomly divided into four groups:model control group(1mL/kg distilled water),drug group(3 mg/kg cisapride),massage group(abdominal massage),drug+massage group(3 mg/kg cisapride+abdominal massage)with 13 in every group;and 13 normal rats were as blank group(1 mL/kg distilled water).Feeding for 10 days,the defecation volume of rats in every group was collected on the first day and the 10th day of modeling,and the 7th day and the 14th day of intervening,the dry wet ratio was calculated and the stress defecation time was recorded.The contents of serotonin(5-HT)and vasoactive intestinal peptide(VIP)in serum were determined by enzyme-linked immunosorbent assay(ELISA).ResultsCompared with the blank group,the defecation volume of rats in other groups decreased,the time of stress defecation prolonged and the ratio of dry to wet defecation increased on the 10th day(P<0.05);the levels of 5-HT and VIP in serum of the model group were lower than that of the control group(P<0.05).Compared with the model group,the defecation volume of the drug group,the massage group and the drug+massage group increased 24 hrs after the intervention,the stress defecation time was shortened,the defecation dry wet ratio was reduced,the serum 5-HT content was increased,and the VIP content was decreased.The differences were statistically significant(P<0.05).Compared with the drug group,the serum VIP content of the drug+massage group decreased significantly on the 14th day after the intervention(P<0.05).Compared with the massage group,the serum 5-HT and VIP contents in the drug+massage group increased and decreased respectively on the 14th day after the intervention,and the differences were statistically significant(P<0.05).ConclusionAbdominal massage has a good therapeutic effect on slow

关 键 词：结肠慢传输型便秘 腹部推拿 五羟色胺 血管活性肠肽 

分 类 号：R245[医药卫生—针灸推拿学]