金属酞菁-血清白蛋白杂化酶的合成及其性质的研究  

Synthesis and characterization of meter phthalocyanines and hybrid proteins

作　　者：赵丹丹 张恩风 莫丹 白丽娟 张玉贞 边贺东 ZHAO Dan-dan;ZHANG En-feng;MO Dan;BAI Li-juan;ZHANG Yu-zhen;BIAN He-dong(School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Guangxi Key Laboratory ofChemistry and Engineering of Forest Products,Nanning 530006,China)

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西南宁530006

出　　处：《应用化工》2020年第3期645-650,共6页Applied Chemical Industry

基　　金：广西自然科学基金资助项目(2016GXNSFDA380005);广西科技基地和人才专项(AD18126005);广西民族大学研究生教育创新计划资助项目(gxun-chxps201820)。

摘　　要：通过熔融法以均苯四甲酸酐、尿素及相应的金属盐为原料,得到八羧基酞菁锰(MnOCPc)和八羧基酞菁铁(FeOCPc)。通过荧光光谱、紫外光谱、圆二色光谱等分析方法研究了金属酞菁与BSA的相互作用。结果表明,BSA的荧光发生了规律性的猝灭,其猝灭类型主要为静态猝灭。通过Stern-Volmer方程得到了两种金属酞菁对BSA的荧光猝灭常数和结合位点数等信息。圆二色光谱表明,金属酞菁使BSA的构象发生了变化,α-螺旋结构含量降低。抗氧化活性测试表明,金属酞菁、金属酞菁-BSA加合物均表现出一定的SOD活性,牛血清白蛋白的加入增强了金属酞菁的SOD活性。The FeOCPc(Fe-octacarboxyl ropathalocyanine)and MnOCPc(Mn-octacarboxyl pathalocyanine)were synthesized using pathalic anhydride,urea and ammoniam molybdate with melting method.The interaction between metal phthalocyanine and BSA was analyzed using fluorescence,UV,and circular dichroism.The results showed that metal phthalocyanines can cause the endogenous fluorescence quenching of BSA,and they are mainly static quenching.The information such as the quenching constant K SV,the binding constant K,and the binding method was calculated through Stern-Volme equation.Circular dichroism showed that the addition of metal phthalocyanine changes the conformation of BSA and decreases the content ofα-helical structure.The antioxidant activity tests of metal phthalocyanine and metal phthalocyanine-BSA adducts showed that FeOCPc and MnOCPc showed some SOD activities,and the addition of BSA can enhannce SOD activities of metal phthalocyanine.

关 键 词：金属酞菁配合物 牛血清白蛋白 相互作用 SOD活性测试 

分 类 号：TQ028[化学工程] TQ25