Graves病患者肠道菌群多样性分析  

Diversity analysis of intestinal microbiota in Graves disease patients

作　　者：孙博文 杨孟雪 刘军 周雪 李飞 杨波 SUN Bowen;YANG Mengxue;LIU Jun;ZHOU Xue;LI Fei;YANG Bo(Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学附属医院内分泌科,贵州遵义563000 [2]复旦大学附属上海市第五人民医院内分泌科,上海200240

出　　处：《实用医学杂志》2020年第6期767-773,共7页The Journal of Practical Medicine

基　　金：贵州省科技厅支撑计划[编号:黔科合支撑[2017]2884];国家自然科学基金项目(编号:81560147);贵州省科技攻关项目[编号:黔科合SY字(2012)3116号];贵州省科学技术基金项目[编号:黔科合J字LKZ(2013)53号]。

摘　　要：目的初步探究Graves病(Graves disease,GD)患者肠道菌群的多样性变化。方法选择遵义医科大学附属医院就诊的初诊Graves病患者(n=18例),同期于体检中心体检的健康人群(n=11例),受试者均排除近1个月内合并感染性疾病、其他自身免疫疾病、应用益生菌及抗生素等。并收集其粪便样本,提取肠道菌群DNA,16S rRNA基因扩增,Illumina平台测序,对测序结果进行物种注释、多样性及物种差异分析。结果测序共测得1200665条高质量序列,每个样本平均含有(41402±6733)条有效序列。2组间Chao1指数、ACE指数、Shannon指数及Simpson指数没有统计学差异(P>0.05),但GD组均小于NC组,表明Graves病患者肠道菌群多样性降低。2组间肠道菌群组成和结构存在显著差异,门水平上GD组厚壁菌门相对丰度低于NC组,而放线菌门高于NC组(P<0.05)。属水平上GD组双歧杆菌属、柯林斯氏菌属、Pediococcus、N09、02d06等相对于NC组优势丰度分布,而罗斯氏菌属、小杆菌属、Thermus、Slackia、[Prevotella]等相对丰度低于NC组(P<0.05)。结论通过对Graves病患者肠道菌群的多样性分析,可得出Graves病患者与正常人肠道菌群确有差异,肠道菌群紊乱导致肠道慢性炎症状态及免疫激活可能是Graves病的发病机制之一。Objective To explore the diversity of intestinal microbiota in patients with Graves disease(GD).Methods 18 newly diagnosed patients with Graves disease in the affiliated Hospital of Zunyi Medical University were selected as the observation group,and 11 healthy people were included in the normal control group.Intestinal microbiota DNA of the fecal samples were extracted,16 S rRNA amplification and Illumine sequencing.Species annotation,diversity and species differenceswere analyzed baced on the sequencing results.Result A total of 1200665 high quality sequences were obtained by sequencing,and each sample contained(41402±6733)valid sequences on average.There were no differences in Chao1 index,ACE index、Shannon index and Simpson index between the two groups(P>0.05),but the diversity of intestinal microbiota in GD group was lower than that in NC group.There was a significant difference in the composition and structure of the intestinal microbiota between the two groups.The relative abundance of Firmicutes in GD group was lower than that in NC group,while Actinobacteria was higher than that in NC group(P<0.05).The relative abundance of Roseburia,Dialister,Thermus,Slackia,[Prevotella],etc in GD group was lower than that in NC group,while Bifidobacterium,Collinsella,Pediococcus,N09,02 d06,etc was higher than that in NC group(P<0.05).Conclusion Through the analysis of the diversity of intestinal microbiota in Graves patients,it can be concluded that there is a difference in intestinal microbiota between Graves patients and normal people.The chronic inflammation and immune activation caused by intestinal microbiota disorder may be one of the pathogenesis of Graves disease.

关 键 词：GRAVES病 肠道菌群 16S rRNA基因测序 Th17/Treg轴 短链脂肪酸 

分 类 号：R378[医药卫生—病原生物学]