灰葡萄孢蛋白质毒素的致病性研究  

Study on Pathogenicity of Protein Toxin Secreted from Botrytics cinerea

作　　者：孔琼[1] 袁盛勇[1] 李珣[1] 薛春丽 李林倩 余朝阳 杨红玉[2] KONG Qiong;YUAN Shengyong;LI Xun;XUE Chunli;LI Linqian;YU Chaoyang;YANG Hongyu(College of Life Science and Technology,Honghe University, Mengzi 661100,China;Kunming University,Kunming 650214,China)

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661100 [2]昆明学院,云南昆明650214

出　　处：《河南农业科学》2020年第4期72-77,共6页Journal of Henan Agricultural Sciences

基　　金：红河学院博士启动项目(14bs14);国家自然科学基金项目(31260062);红河学院硕士点植物保护一级学科建设项目。

摘　　要：灰葡萄孢是一种危害较大的植物病原菌,为了解灰葡萄孢致病因子和其毒素的寄主选择性,以分离于番茄灰葡萄孢分泌的蛋白质毒素为侵染源,对16科23种供试植物叶片和7种供试植物种子进行致病性和寄主专化性测定。结果发现,灰葡萄孢分泌的蛋白质毒素能侵染17种植物叶片,占全部供试植物的73.91%。对石蒜科朱顶红、茄科辣椒叶片的致病性最强,7 d后的病斑面积分别为(2.18±0.44)、(0.81±0.33)cm^2,其次分别是五加科的花叶鸭脚木、菊科的菊花和莴苣、茄科的番茄和烟草、豆科的蚕豆和豌豆。灰葡萄孢分泌的蛋白质毒素能明显抑制十字花科的大白菜、豆科的蚕豆和豌豆、茄科的番茄等7种供试植物种子的萌发和胚芽生长。综上,灰葡萄孢寄主范围广,其分泌的蛋白质毒素有毒性,且为非寄主专化性毒素。Botrytis cinerea was a serious pathogen for plants.In order to provide the reference for studying pathogenic mechanism and control measures of Botrytis cinerea,the pathogenicity and host speciality of protein toxin secreted from Botrytics cinerea were determined with plants from 23 species in 16 families.The results showed that 17 species of plant were damaged by protein toxin,accounting for 73.91%of total plants,the pathogenicity of the toxin was stronger to Hippeastrum rutilum of Amaryllidaceae and Capsicum annuum of Solanaceae,and the lesion areas were respectively(2.18±0.44),(0.81±0.33)cm^2 after 7 days of inoculation,followed by Schefflera octophylla of Araliaceae,Dendranthema morifolium and Lactuca sativa of Compositae,Lycopersicon esculentum and Nicotiana tabacum of Solanaceae,Vicia faba and Pisum sativum of Leguminosae.The protein toxin could significantly inhibit seed germination and plantule growth of seven plants,such as Brassica pekinensis of Cruciferae,Vicia faba and Pisum sativum of Leguminosae,Lycopersicon esculentum of Solanaceae.Therefore,the host of Botrytis cinerea was wide,and its protein toxin was pathogenic with a non-host specificity.

关 键 词：灰葡萄孢 蛋白质毒素 致病性 离体接种 寄主专化性 

分 类 号：S432.4[农业科学—植物病理学]