香草酸对感染性结石大鼠尿路组织的病理学影响  

Pathological effects of vanillic acid on urinary tract in rats with infectious calculi

作　　者：关帅 郝川[1] 李承勇[1] GUAN Shuai;HAO Chuan;LI Cheng-yong(Department of Urology, Second Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, China)

机构地区：[1]山西医科大学第二医院泌尿外科,山西太原030001

出　　处：《海南医学院学报》2020年第8期571-574,共4页Journal of Hainan Medical University

基　　金：山西省科技发展计划(社会发展)项目(20150313009-3)。

摘　　要：目的:通过构建感染性大鼠结石模型,观察香草酸治疗后大鼠尿路组织的病理改变。方法:雄性Wistar大鼠40只,随机分为空白对照组、感染性结石组、香草酸组及乙酰氧肟酸组。空白对照组4只,不予特殊处理,常规饲养。感染性结石组12只,将染有奇异变形杆菌的聚氨酯静脉留置针前端4 mm含菌异物植入膀胱,诱导形成感染性膀胱结石模型。香草酸组12只,建立感染性膀胱结石模型后,香草酸灌胃50 mg·kg^-1·d^-1,连续3周。乙酰氧肟酸组12只,建立感染性膀胱结石模型后,乙酰氧肟酸12.5 mg·kg^-1·d^-1灌胃。21 d后处死大鼠,将肾脏、输尿管和膀胱组织置入10%多聚甲醛液中固定24 h,常规石蜡包埋标本,HE及免疫组化染色后进行相关的病理学分析。结果:除空白对照组外,其余3组大鼠膀胱内均有结石生成。空白对照组肾脏、输尿管、膀胱未见明显病理改变,其余3组均有不同程度炎症反应:感染性结石组表现为严重的尿路炎症,乙酰氧肟酸次之。香草酸组尿路组织病变最轻,表现为尿路组织少量炎细胞浸润及黏膜增生,未见黏膜脱失等严重病理改变。与感染性结石组及乙酰氧肟酸组相比,香草酸组肾小管亨利袢升支粗段和远曲小管近段THP蛋白表达不明显。结论:奇异变形杆菌及其所形成的感染性结石可导致尿路上皮损伤,香草酸抑制感染性结石的同时能减轻了尿路组织的炎性病变。Objective The pathological changes of urinary tract were observed after the treatment of infectious stone by vanillic acid.MethodsTotally 40 male Wistar rats were randomly divided into control group,infectious stone group,vanillic acid group and acetohydroxamic acid group.In control group 4 male Wistar rats were fed without special treatment while in infectious stone group 12 male Wistar rats were implanted with 4 mm bacteria bearing foreign body in the front of the polyurethane vein indwelling needle infected with Proteus mirabilis into the bladder to induce the formation of infection stone.In the vanillic acid group,after the model were establishmented,12 male Wistar rats were treated with vanillic acid at 50 mg·kg^-1·d^-1 for 3 weeks.In acetohydroxamic acid group 12 male Wistar rats were treated with acetohydroxamic acid at 12.5 mg·kg^-1·d^-1 for 3 weeks after the model were establishmented.The rats were killed 21 d later.The kidney,ureter and bladder were fixed in 10%paraformaldehyde solution for 24 h.The specimens were embedded in paraffin,and the pathological analysis was carried out after HE and immunohistochemical staining.Results In addition to the control group,the other three groups of rats had calculus formation in the bladder.No obvious pathological changes were found in the kidney,ureter and bladder in the control group,and inflammatory reactions were found in the other three groups severe urinary tract inflammation was found in the infectious stone group,followed by acetylhydroxamic acid.In the vanillic acid group,the pathological changes of urinary tract were the lightest,with a small amount of inflammatory cell infiltration and mucosal hyperplasia,but no serious pathological changes such as mucosal loss.The expression of Tamm-Horsfall Protein(THP)in the ascending branch of loop of Henle and the proximal part of distal tubule in vanillic acid group was not significant compared with that in the infectious stone group and acetohydroxamic acid group.Conclusion Proteus mirabilis and its infective

关 键 词：奇异变形杆菌 香草酸 乙酰氧肟酸 结石 感染 病理 

分 类 号：R691.4[医药卫生—泌尿科学]