前列消癥汤对前列腺癌DU-145细胞凋亡及周期的影响  

Effects of QianlieXiaozheng Decoction on Apoptosis and Cell Cycle of DU-145 Cells

作　　者：祁雷磊[1] 张丽娟 周冰莹 宋竖旗[1] 卢建新[1] 尹学来[1] 庞然[1] QI Lei-lei;ZHANG Li-juan;ZHOU Bing-ying(Department of Urology,Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing(100053),China)

机构地区：[1]中国中医科学院广安门医院泌尿外科,北京100053

出　　处：《中国中西医结合外科杂志》2020年第2期226-231,共6页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：北京市自然科学基金(7112119、7112118);北京市科学技术委员会资助(Z161100000516156)。

摘　　要：目的:观察前列消癥汤对前列腺癌DU-145细胞系的细胞凋亡及周期的影响,探讨前列消癥汤治疗前列腺癌的生物学机制。方法:采用流式细胞术测定不同浓度前列消癥汤对前列腺癌DU-145细胞凋亡及细胞周期分布的影响。结果:前列消癥汤在一定浓度范围内抑制DU-145细胞增殖,并呈现出一定的量效关系。与对照组比较,中、高剂量组均能够显著增加早期凋亡、晚期凋亡,具有统计学差异(中剂量组P<0.05,高剂量组P<0.01)。中剂量与高剂量组的S期细胞比例分别为(37.96±2.24)%和(43.77±2.48)%,均显著高于对照组的(31.69±1.29)%,差异有统计学意义(中剂量组P<0.05,高剂量组P<0.01)。结论:前列消癥汤能够诱导前列腺癌DU-145细胞的早期及晚期凋亡,并随着剂量的增加,促进凋亡的作用也随着增加。前列消癥汤主要抑制前列腺癌DU-145细胞从S期向G2期的转变。Objective To observe the effect of QianlieXiaozheng Decoction(QXD)on the cell cycle and apoptosis of DU-145 cells.Methods The cell cycle and apoptosis of DU-145 cells under different concentration of QXDwere researchedby flowcytometry.Results The early stage of apoptosisandlate stageof apoptosis in medium dose group both had statistical differences compared with control group(P<0.05).The early stage of apoptosisandlate stageof apoptosis in high dose group both had statisticallysignificantdiff erences compared with control dose group(P<0.01).The result of cell cycle showed that the ratio of cells in S period in control group and medium dose group was(31.69±1.29)%and(37.96±2.24)%respectively,with statistically significant difference(P<0.05).The ratio of cells in S period in control group and high dose group was(31.69±1.29)%and(43.77±2.48)%respectively,with statistically significantdifference(P<0.01).Conclusion QXD could induce the apoptosis of early stage and late stage of DU-145 cells.The induction augmented which was followed by the concentration increasing.The results predicted that the QXD could stop the cell cycle of DU-145 cells and turn S period into G2 period.

关 键 词：DU-145细胞 前列消癥汤 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R737.25[医药卫生—中医学]