胎牛血清对猪精子常温负压保存质量的影响  

Effect of Fetal Bovine Serum on the Quality of Porcine Sperm under Normal Temperature and Negative Pressure

作　　者：李井春[1] 王硕 李雁冰[1] 王国鑫[1] 魏国生[1] Li Jingchun;Wang Shuo;Li Yanbing;Wang Gouxin;Wei Guosheng(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2020年第2期27-31,67,共6页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省自然科学基金面上项目(C2017053);黑龙江省农垦总局科研计划项目(HNK135-04-06);黑龙江八一农垦大学青年创新人才项目(CXRC2017004);黑龙江八一农垦大学博士启动基金(XYB-2016-04);黑龙江省博士后科研启动基金(LBH-Q18100)。

摘　　要：为探究胎牛血清(FBS)在17℃负压环境下对猪精液的保存效果,延长猪精液保存的时间以及维持猪精子的质量。通过在Modena基础稀释液中分别添加体积分数为0、5%灭活、10%灭活、非灭活5%、非灭活10%的FBS,检测猪精子在此环境下5 d内的精子活率、直线速度、侧摆幅度等为检测参数。结果表明:10%灭活的FBS在猪精子各项参数上都显著优于其他组(P<0.05)。由结果可知,在Modena基础稀释液添加10%灭活的FBS在17℃负压环境下最有利于猪精液的保存,为猪精子的常温保存研究提供实验依据。To explore the effect of fetal bovine serum(FBS)on the preservation of pig semen under 17℃negative pressure environment,prolong the preservation time of pig semen and maintain the quality of pig sperm,adding the volume fraction in Modena foundation diluent respectively 0,5%inactivated,10%inactivated,5%lived,and 10%lived FBS,detect the pig sperm in the environment within the 5 d’s live sperm rate,linear velocity,side swing range,etc.The results showed that 10%inactivated FBS was significantly better than other groups in various parameters of pig sperm(P<0.05).The result showed that in Modena diluent adding 10%inactivated FBS in 17℃under negative pressure environment most conducive to the pig semen preservation,which would provide experiment basis for the research on the pig sperm normal temperature preservation.

关 键 词：胎牛血清 猪精子 17℃ 负压 

分 类 号：S828[农业科学—畜牧学]