超高效液相色谱-串联质谱法检测动物源性食品中的β-受体激动剂类和喹诺酮类物质  

Application of ultra high performance liquid chromatography-tandem mass spectrometry in determination of β-agonists and quinolones in foodstuff of animal origin

作　　者：刘新辉 马小岭 季秋红 LIU Xinhui;MA Xiaoling;JI Qiuhong(Shandong Baier Testing Co.Ltd.,Weifang 261000,China;Shandong Artisan Testing Co.Ltd.,Weifang 261000,China)

机构地区：[1]山东拜尔检测股份有限公司,山东潍坊261000 [2]山东匠造检测有限公司,山东潍坊261000

出　　处：《邵阳学院学报（自然科学版）》2020年第2期55-66,共12页Journal of Shaoyang University：Natural Science Edition

摘　　要：目的建立了超高效液相色谱-串联质谱(UHPLC-MS/MS)法检测畜肉及其副产品中β-受体激动剂类和喹诺酮类物质的测定方法。方法采用1%乙酸水溶液质量分数水解试样,将残留物从轭合物形态转化为游离形式,经乙腈提取、Cleanert LipoNo净化后,ZORBAX Eclipse Plus C18色谱柱分离,经超高效液相色谱-串联质谱仪检测,内标法定量。结果12种β-受体激动剂及11种喹诺酮在质量浓度为0.5~20 Hg/L范围内线性关系良好(R^2≥0.99),平均回收率为80.2%~106.0%,相对标准偏差均小于9.52%。结论方法操作简便,成本低,有机溶剂使用量少且稳定性高,能够为政府监管及畜肉企业内部质控提供技术支持。Objective A method was developed for the simultaneous determination of β-agonists and quinolones in foodstuff of animal origin by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Methods The samples were hydrolysised with 1%acetic acid solution,the residue was converted from conjugate form to free form,and extracted with acetonitrile.The free β-agonists and quinolones were purified using Cleanert LipoNo,separated on a ZORBAX Eclipse Plus C18 column,and detected with UHPLC-MS/MS by internal standard method.Results The average recoveries for the target compounds were between 93.2%-108% with RSDs less than 5.0%.The linearities of twenty-three compounds were in the concentration ranges of 0.5-20 Hg/L,with the good correlation coefficients(R≥0.99).Conclusion The method has advantages of easy operation,low cost、low use of organic solvents and high stability.The developed method can provide strong technical support for enterprise quality control and government regulation.

关 键 词：β-受体激动剂 喹诺酮 超高效液相色谱-串联质谱 Cleanert LipoNo 

分 类 号：N33[自然科学总论]