冷冻生殖细胞和组织用于保存实验动物遗传资源  

Freezing Germ Cells and Tissues to Preserve Laboratory Animal Genetic Resources

作　　者：彭旭 张晓梅 何学令 刘艳 PENG Xu;ZHANG Xiaomei;HE Xueling;LIU Yan(Laboratory Animal Centre,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学实验动物中心,成都610065

出　　处：《实验科学与技术》2020年第2期26-31,共6页Experiment Science and Technology

基　　金：四川大学实验技术立项项目(20170211)。

摘　　要：动物生殖细胞(卵子、胚胎、精子)冷冻保存技术在畜牧生产、临床医学及生物技术等领域发挥了重要作用。利用超低温冷冻保存方法,将珍稀濒危动物和转基因动物生殖细胞保存起来,可实现动物种质资源的长期保存;但是精子和胚胎冷冻技术在疾病模型动物资源保存应用上仍存在许多问题。该文针对拟保存的动物品系生殖细胞和组织在低温冷冻保存中的实际问题及选择进行阐述,以期推进冷冻保存技术在动物资源保存中的应用。Animal germ cell(oocyte,embryo,sperm)cryopreservation technology has played an important role in animal husbandry,clinical medicine and biotechnology.Using ultra-low temperature cryopreservation methods to preserve germ cells of rare and endangered transgenic animals can achieve long-term preservation of animal germplasm resources.However,the cryopreservation of germ cells and tissues of disease animals still have many problems.This article describes the practical problems and selection of germ cells and tissues of animal strains to be preserved in cryopreservation,in order to promote the application of cryopreservation technology in animal resource preservation.

关 键 词：遗传资源 冷冻保存 卵母细胞 胚胎 干细胞 卵巢 

分 类 号：TP75[自动化与计算机技术—检测技术与自动化装置]