BioID技术在肿瘤蛋白质组学中的发展与应用  

Development and Application of Bio ID Technology in Tumor Proteomics

作　　者：王晓飞 丁明 WANG Xiaofei;DING Ming

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《科技创新与应用》2020年第13期164-166,170,共4页Technology Innovation and Application

摘　　要：蛋白质通过形成复合物或蛋白-蛋白相互作用来执行生物学功能,蛋白-蛋白相互作用是细胞生命活动的基础,也是整个生物学领域研究的核心问题之一。传统的互作蛋白筛选方法包括酵母双杂交和串联亲和纯化等,但这些方法具有一定的局限性,很难适用于实时和动态蛋白相互作用分析。邻近依赖生物素鉴定(BioID,proximity-dependent biotin identification)是筛选活细胞中发生的蛋白相互作用的独特方法,利用修饰过的生物素连接酶BirA与感兴趣的蛋白融合表达,生物素化近端内源蛋白质,生物素化的蛋白可以被选择性分离并用生物质谱鉴定出目标蛋白的候选相互作用物。BioID已成功应用于不溶性蛋白及瞬时或弱相互作用蛋白的研究,也逐渐应用于肿瘤等疾病发生机制及药物靶点筛选的研究。文章针对该技术进行了详细总结,并讨论了其在肿瘤蛋白质组学中的应用。Proteins perform biological functions by forming complexes or protein-protein interactions.Protein-protein interaction is not only the basis of cell life activities,but also one of the core issues in the whole biological field.Traditional protein screening methods include yeast two-hybrid and tandem affinity purification,but these methods have some limitations and are difficult to be applied to real-time and dynamic protein interaction analysis.Proximity-dependent biotin identification(BioID)is a unique method for screening protein interactions in living cells.Using the fusion expression of modified biotin ligase BirA with proteins of interest,biotinylated proximal endogenous proteins and biotinylated proteins can be selectively isolated and candidate interactions of target proteins can be identified by mass spectrometry.BioID has been successfully applied to the study of insoluble proteins and transient or weakly interacting proteins,and also gradually applied to the pathogenesis of tumors and other diseases as well as drug target screening.In this paper,this technique is summarized in detail,and its application in tumor proteomics is discussed.

关 键 词：BioID 邻近蛋白标记 蛋白-蛋白相互作用 

分 类 号：O629.73[理学—有机化学]