广州市香港海鸥型菌红霉素耐药性与基因分析  

Resistance and genetic analysis of erythromycin in Laribacter Hongkongensis in Guangzhou

作　　者：张铮峰 张冰纯 王盼盼 陈定强[2] 赵路宁[1] 余琳[2] ZHANG Zeng-feng;ZHANG Bing-chun;WANG Pan-pan;CHEN Ding-qiang;ZHAO Yu-ling;YU Lin(Guangzhou Medical University,Guangzhou 510080,China;The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China)

机构地区：[1]广州医科大学,广州510080 [2]广州医科大学附属第一医院,广州510120

出　　处：《中国人兽共患病学报》2020年第4期309-312,共4页Chinese Journal of Zoonoses

基　　金：2018年广东省自然科学基金(No.2018A030313279);2018年广州医科大学大学生实验室开放项目;2019-2020年度大学生科技创新项目(No.2018A091)共同资助。

摘　　要：目的了解广州地区香港海鸥型菌分离株对红霉素的耐药性及红霉素耐药基因的分布情况。方法从青蛙和草鱼肠道中分离的菌株共122株,使用K-B法对香港海鸥型菌分离株进行红霉素抗生素的耐药性测定,用PCR方法检测红霉素耐药株中红霉素抗性基因ereA,ereB,ermA,ermB,ernC,MefA/E,mphA,SAT4的携带情况,并将检测阳性结果的PCR产物进行测序分析。结果香港海鸥型菌对红霉素耐药率为28.7%,其中蛙源株耐药率为35.1%,鱼源株耐药率为25.9%。对红霉素耐药的35株菌中,蛙源株ereA基因检出率为30.8%,鱼源株没有检出ereA基因。结论广州地区香港海鸥型菌对红霉素的耐药率较高,蛙源株对红霉素的耐药率比鱼源株高,海鸥型菌蛙源株对红霉素产生的耐药性与ereA基因相关。To understand the resistance of Laribacter hongkongensis(LH) to erythromycin and the distribution of erythromycin resistance genes in Guangzhou. The strains were collected from the Department of Clinical Laboratory of the First Affiliated Hospital of Guangzhou Medical University, 122 strains were isolated from the intestines of frog and grass carp. The erythromycin antibiotic resistance of LH was determined by KB method. The erythromycin resistance genes ereA, ereB, ermA, ermB, ernC, MefA/E, mphA, and SAT4 were detected by PCR. The PCR products with positive results were subjected to sequencing analysis. The resistance rate of LH to erythromycin was 28.7%, among which the resistance rate of frog source strain was 35.1%, and that of fish source strain was 25.9%. Among the 35 strains resistant to erythromycin, the ereA gene detection rate was 30.8% in frog source strain, while the ereA gene was not detected in the fish source strain. These results iudicated that the erythromycin resistance rate of LH is high in Guangzhou. The erythromycin resistance rate of frog source is higher than that of fish source. The erythromycin resistance of LH from frog is related to the ereA gene.

关 键 词：香港海鸥型菌 红霉素 耐药性 耐药基因 

分 类 号：R378[医药卫生—病原生物学]