高效液相色谱-荧光检测法同时测定化妆品中的α-熊果苷、β-熊果苷、氢醌和苯酚  

Determination ofα-arbutin,β-arbutin,hydroquinone and phenol in cosmetics by high performance liquid chromatography-fluorescent detection

作　　者：简龙海[1] 袁晓倩 郑荣[1] 王柯[1] JIAN Long-hai;YUAN Xiao-qian;ZHENG Rong;WANG Ke(Shanghai Institute for Food and Drug Control,NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics,Shanghai 201203,China)

机构地区：[1]上海市食品药品检验所国家药品监督管理局化妆品监测评价重点实验室,上海201203

出　　处：《药物分析杂志》2020年第2期273-277,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定化妆品中α-熊果苷、β-熊果苷、氢醌和苯酚的高效液相色谱-荧光检测(HPLCFLD)法。方法:样品用甲醇提取,提取液采用C18柱(250 mm×4.6 mm×5μm)分离,以甲醇-水为流动相梯度洗脱,流速为0.5 mL·min-1,进样量5μL,采用荧光检测器检测。结果:α-熊果苷、β-熊果苷、氢醌和苯酚质量浓度分别在0.25~20、0.25~20、0.025~2和0.05~4μg·m L-1范围内线性关系良好,方法检出浓度分别为2、2、0.1和0.2μg·g-1。4个待测物的方法回收率平均为90.0%~96.7%,RSD(n=6)为1.3%~4.4%。测定了54批化妆品样品,均未检出氢醌和苯酚;17批样品检出β-熊果苷,含量在8~49000μg·g-1之间;1批样品检出α-熊果苷,含量为410μg·g-1。结论:本方法准确、灵敏,可用于化妆品中α-熊果苷、β-熊果苷、氢醌和苯酚的测定。Objective:To establish a high performance liquid chromatography-fluorescent detection(HPLCFLD)method for the simultaneous determination ofα-arbutin,β-arbutin,hydroquinone and phenol in cosmetics.Methods:Samples were extracted by methanol,then the extract was separated on a C18 column(250 mm×4.6 mm×5μm)with the mobile phase of methanol-water in gradient elution at a flow rate of 0.5 mL·min-1.The injection was 5μL.The 4 analytes were finally detected by fluorescent detector.Results:The linear relationships ofα-arbutin,β-arbutin,hydroquinone and phenol all showed good in the range of 0.25-20μg·mL-1,0.25-20μg·mL-1,0.025-2μg·mL-1 and 0.05-4μg·mL-1,respectively.The limits of detection were 2,2,0.1 and 0.2μg·g-1,respectively.The average recoveries were in the range of 90.0%-96.7%with the RSDs of 1.3%-4.4%(n=6).A number of fifty-four cosmetic samples were determined.No hydroquinone and phenol were detected.β-arbutin was found in seventeen cosmetic samples with the contents from 8μg·g-1 to 49000μg·g-1.α-arbutin was detected in only one cosmetic sample with the content of 410μg·g-1.Conclusion:The method is accurate and sensitive for the determination ofα-arbutin,β-arbutin,hydroquinone and phenol in cosmetics.

关 键 词：高效液相色谱-荧光检测法 化妆品 熊果苷 氢醌 苯酚 

分 类 号：R917[医药卫生—药物分析学]