多烯紫杉醇石墨烯的制备及质量评价  

Preparation and quality evaluation of functional graphene oxied/docetaxel

作　　者：申玉坤[1,2] 申玉璞[1] 潘一峰[2] 石春花[1] SHEN Yu-kun;SHEN Yu-pu;PAN Yi-feng;SHI Chun-hua(Changzhi Medical College,Changzhi 046000,China;National Key Laboratory of Nanobiological Technology,Ministry of Health,Xiangya Medical College,Central South University,Changsha 410008,China)

机构地区：[1]长治医学院,长治046000 [2]中南大学湘雅医学院卫生部纳米生物技术重点实验室,长沙410008

出　　处：《药物分析杂志》2020年第2期388-391,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(81302075);长治医学院科研启动基金项目(QDZ201629);长治医学院博士启动基金(BS15015)。

摘　　要：目的:合成新型的多烯紫杉醇石墨烯复合材料(Fe3O4/GO-NA/DOC),并对其进行质量评价。方法:利用透射电镜观察其形貌;以高效液相色谱法(HPLC)测定多烯紫杉醇(DOC)的含量,并对生成物的包封率、回收率以及精密度进行评价,以低温高速离心法测定Fe3O4/GO-NA/DOC复合材料的包封率。结果:多烯紫杉醇检测浓度线性范围为20~120μg·mL-1(r=0.9991),平均回收率(n=3)为100.2%±0.23%,在转速为20000 r·min-1,离心时间为80 min的条件下,包封率的值在90%以上。结论:Fe3O4/GO-NA/DOC复合材料制备工艺可行,质量控制方法简便可靠。Objective:To synthetize the new-type Fe3 O4/GO-NA/DOC composite materials,and evaluate their qualities.Methods:The morphology was observed by transmission electron microscope.The concentration of DOC was determined by high efficiency liquid chromatography.The recovery,precision and entrapment efficiency of Fe3 O4/GO-NA/DOC composite materials were studied,and the Fe3 O4/GO-NA/DOC composite materials entrapment efficiency was determined by Low temperature high speed centrifugation.Results:The linear detection concentration range of DOC was 20-120μg·mL-1(r=0.9991)with the average recovery(n=3)of 100.2%±0.23%.At a speed of 20000 r·min-1 and a centrifugation time of 80 min,the encapsulation rate was more than 90%.Conclusion:The preparation technique of Fe3 O4/GO-NA/DOC composite materials is feasible,and the quality control method is convenient and reliable.

关 键 词：多烯紫杉醇 石墨烯复合材料 包封率 高效液相色谱法 质量评价 

分 类 号：R917[医药卫生—药物分析学]