壳聚糖纳米颗粒对红鳍东方鲀生长、免疫酶及免疫基因的影响  

Effects of chitosan nanoparticles on the growth,immune enzyme,and genes of tiger puffer Takifugu rubripes

作　　者：王荣月 劳锦程 王重阳 李睿鑫 谢红国[2] 叶仕根[1] 黎睿君[1] 尹恒[2] 刘娟 WANG Rongyue;LAO Jincheng;WANG Chongyang;LI Ruixin;XIE Hongguo;YE Shigen;LI Ruijun;YIN Heng;LIU Juan(Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture and Rural Affairs,Dalian Key Laboratory of Marine Animal Disease Prevention and Control,Dalian Ocean University,Dalian 116023,China;Natural Products and Glyco-Biotechnology,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China)

机构地区：[1]大连海洋大学农业农村部北方海水增养殖重点实验室,大连市海珍品疾病预防重点实验室,辽宁大连116023 [2]中国科学院大连化学物理研究所天然产物及糖工程研究组,辽宁大连116023

出　　处：《大连海洋大学学报》2020年第2期177-183,共7页Journal of Dalian Ocean University

基　　金：辽宁省高等学校海洋产业技术研究院项目(2018-CY-13);农业农村部北方海水增养殖重点实验开放课题(2018-KF-19);大连海洋大学大学生创新创业训练计划项目(201810158067);大连海洋大学引进人才启动项目(HDYJ201812)。

摘　　要：为了研究壳聚糖纳米颗粒这一新型免疫添加剂对红鳍东方鲀Takifugu rubripes生长性能、免疫指标的影响,以体质量为(9.910±0.306)g的红鳍东方鲀幼鱼为研究对象,试验设6组,每组设3个平行,每个平行放30尾鱼,投喂基础饲料中分别添加0(对照)、0.2%的壳寡糖(COS)、0.2%的壳聚糖(CS),以及0.2%、0.1%、0.02%的壳聚糖纳米颗粒(CN)的试验饲料,养殖周期50 d,试验结束后测定各组红鳍东方鲀的特定生长率,肝、脾、肾组织中碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)及溶菌酶(LZM)的活性,以及补体c3、热休克性蛋白hsp70、免疫调节因子ifr2、Toll样受体tlr3等免疫基因的相对表达量。结果表明:饲料中添加壳聚糖纳米颗粒和壳寡糖能显著促进红鳍东方鲀的生长(P<0.05);饲料中添加壳聚糖纳米颗粒能显著增加鱼体肝脏的AKP、CAT酶活性,脾脏的ACP、LZM活性,肾脏的SOD、LZM酶活性(P<0.05);壳聚糖纳米颗粒对鱼体ifr2基因表达的促进作用不明显(P>0.05),但能显著提高脾脏中c3、tlr3、hsp70的相对表达量(P<0.05)。研究表明,饲料中添加壳聚糖纳米颗粒对红鳍东方鲀的生长具有促进作用,并能提高鱼体的免疫力,同时与壳寡糖的作用效果相当。Juvenile tiger puffer Takifugu rubripes with body weight of(9.910±0.306)g were fed diets containing 0%(control group),and 0.2%of chitosan oligosaccharide(COS),0.2%of the chitosan(CS),and 0.2%,0.1%,and 0.02%of the chitosan nanoparticles(CN)at water temperature of 17-23℃for 50 d,and activities of alkaline phosphatase(AKP),superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP)and lysozyme(LZM)were determined and the relative expression levels of c3,hsp70,ifr2 and tlr3 genes were analyzed in liver,spleen and kidney by real-time quantitative PCR at the end of the experiment in order to investigate the effect of chitosan nanoparticles,a new immunopotentiator,on the growth performance and immune of tiger puffer.The results showed that chitosan nanoparticles led to significantly promote the growth and to increase in activities of AKP and CAT in liver,ACP and LZM in spleen,and SOD and LZM in kidney of tiger puffer(P<0.05).There were significant increase in the relative expression levels of c3,tlr3 and hsp70 genes in spleen in the tiger puffer fed the diets containing chitosan nanoparticles(P<0.05),without significant improvement of the relative expression of ifr2 gene(P>0.05).It was concluded that dietary chitosan nanoparticles resulted in improvement of the growth performance and immunity of tiger puffer,and its efficacy was equivalent with the chitosan oligosaccharide additive.

关 键 词：红鳍东方鲀 壳聚糖纳米颗粒 生长性能 酶活性 实时定量荧光PCR 

分 类 号：S917[农业科学—水产科学]