黄淮北片冬麦区抗赤霉病基因Fhb1种质挖掘及溯源  

Screening of Fhb1 Germplasms Resistant to Fusarium Head Blight and Its Putative Ancestor in North of Huang and Huai Winter Wheat Region

作　　者：张颖君[1] 高慧敏[2] 李子千[1] 胡梦芸[1] 孙丽静[1] 刘茜[1] 吕亮杰 李辉[1] ZHANG Yingjun;GAO Huimin;LI Ziqian;HU Mengyun;SUN Lijing;LIU Qian;L Liangjie;LI Hui(Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Hebei Provincial Laboratory of Crop Genetics and Breeding,Shijiazhuang 050035,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北石家庄050035 [2]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《华北农学报》2020年第2期196-202,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家重点研发计划课题(2018YFD0300503);河北省农林科学院创新工程课题(4-08-03)。

摘　　要：小麦赤霉病是一种危害性很强的小麦真菌病害,而Fhb1是抗赤霉病的主效基因。为了筛选黄淮冬麦区携带Fhb1基因的种质材料,利用TaHRC-Kasp和His-InDel标记对来自河北、河南、山东、陕西、江苏和安徽的共336份小麦材料进行检测,共筛选出2份来自河北省的材料(石家庄75和紫茎白)携带Fhb1基因。通过序列分析,其His基因序列与苏麦3号一致,为抗赤霉病类型。利用9 779个SNP位点对供试的336份材料进行PCA主成分分析,结果显示,石家庄75和紫茎白与江苏的材料亲缘关系较近。石家庄75是建国初期的育成品种,紫茎白是河北省的地方农家种,其携带Fhb1基因,说明最初Fhb1基因可能在国内一些地方品种中有所分布,但在现代育种进程中受到人工选择而消失。研究结果为黄淮冬麦区抗赤霉病小麦育种提供了宝贵的遗传信息及亲本材料。Fusarium head blight(FHB)is one of the most destructive diseases in wheat,and Fhb1 is the main gene for FHB resistance. In order to screen the germplasms carrying Fhb1 gene in Huang and Huai winter wheat region,336 wheat cultivars from Hebei,Henan,Shandong,Shaanxi,Jiangsu and Anhui were detected by TaHRC-Kasp and His-InDel markers. Two cultivars(Shijiazhuang 75 and Zijingbai)from Hebei Province were screened to carry Fhb1 gene. Sequence analysis showed that the His gene sequences of these two cultivars were same as that of Sumai 3(FHB resistance type). Principal component analysis(PCA)of 336 materials was performed using 9 779 SNP loci and showed that Shijiazhuang 75 and Zijingbai had close genetic relationship with Jiangsu materials. Shijiazhuang 75 was bred in the early days of the founding of China. Zijingbai was a landrace in Hebei Province. These two materials carried Fhb1 gene,which indicated that Fhb1 gene might be distributed in some local germplasms in China at first. But it was eliminated under artificial selection pressure in the process of modern wheat breeding. The results of this study provided valuable genetic information and parent materials for FHB-resistant wheat breeding in Huang and Huai winter wheat region.

关 键 词：小麦 黄淮冬麦区 赤霉病 Fhb1基因 种质挖掘 

分 类 号：S512.01[农业科学—作物学] Q78[生物学—分子生物学]