克里本类芽孢杆菌PS04诱导水稻抗病相关基因的表达分析  

Expression Analysis of Disease Resistance-related Genes in Rice Plant Induced by Paenibacillus kribbensis PS04

作　　者：程妍 曲玮茵 郑文博 杨媚 廖美德 周而勋 舒灿伟 CHENG Yan;QU Weiyin;ZHENG Wenbo;YANG Mei;LIAO Meide;ZHOU Erxun;SHU Canwei(College of Agriculture,South China Agricultural University,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrative Microbiology Research Center,Guangzhou 510642,China)

机构地区：[1]华南农业大学农学院,广东省微生物信号与作物病害防控重点实验室,群体微生物研究中心,广东广州510642

出　　处：《华北农学报》2020年第2期203-209,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家重点研发专项(2018YFD0200300)。

摘　　要：克里本类芽孢杆菌PS04是一个水稻纹枯病的高效生防菌,为研究该菌诱导水稻抗性相关基因的表达谱,用PS04菌株发酵液处理水稻24 h后于3,6,9,12,24 h取样,利用荧光定量PCR检测样品中抗性相关基因的相对表达量。结果如下:PS04菌株发酵液能够诱导8个防卫反应基因(OsPR1、OsPR2、OsPR3、OsPR4、OsPR5、OsPR10、OsPR16和NPR1)、2个水杨酸途径基因(PAL和OsNAC4)和3个茉莉酸/乙烯途径基因(AOS2、LOX和EIN2)的表达。其中NPR1和LOX基因在第3小时表达量达最高随后下降;OsPR3、OsPR4、OsPR5、OsPR16和PAL基因在第6小时表达量达最高随后下降;OsPR1和OsNAC4基因在第9小时表达量达最高随后下降;OsPR10基因在第12小时表达量达最高随后下降;OsPR2和EIN2基因在第24小时表达量达到最大值;AOS2基因的表达受抑制,在第6小时表达量达最高随后下降。结果表明,PS04菌株可以同时诱导水稻系统性获得抗性和诱导性系统抗性。Paenibacillus kribbensis PS04 is a high-efficiency bacterium for the biocontrol of rice sheath blight. In order to investigate the expression profiles of various disease resistance-related genes,rice leaves were harvested at 3,6,9,12,24 h after 24 h sprayed with fermentation liquid of PS04 strain. Real-time PCR analysis were performed to monitor the expression levels of rice disease resistance-related genes after treatment with PS04 strain. The fermentation liquid of PS04 strain could induce the expression of defense related genes(OsPR1,OsPR2,OsPR3,OsPR4,OsPR5,OsPR10,OsPR16 and NPR1),salicylic acid pathway genes(PAL and OsNAC4),jasmonic acid and ethylene pathways genes(AOS2,LOX and EIN2). The expression of NPR1 and LOX genes reached a maximum level at 3 h and then decreased. The expression of OsPR3,OsPR4,OsPR5,OsPR16 and PAL genes reached a maximum level at 6 h and then decreased. The expression of OsPR1 and OsNAC4 genes reached a maximum level at 9 h and then decreased. The expression of OsPR10 genes reached a maximum level at 12 h and then decreased. The expression of OsPR2 and EIN2 genes reached a maximum level at 24 h. The expression of AOS2 gene was inhibited,and the expression reached the highest level at 6 h and then decreased. The result of this study showed that the PS04 strain could induce both systemic acquired resistance and induced systemic resistance in rice plant.

关 键 词：水稻 类芽孢杆菌 抗病相关基因 诱导抗病性 生物防治 

分 类 号：S432.23[农业科学—植物病理学]