电针“内关”穴对慢性心肌缺血大鼠心交感神经活动及心功能的影响  

Electroacupuncture at “Neiguan”(PC6) decreased cardiac sympathetic hyperactivity and improved cardiac function in chronic myocardial ischemia model rats

作　　者：崔翔[1] 曹洪垒 刘群 刘坤[1] 高昕妍[1] 朱兵[1] CUI Xiang;CAO Hong-lei;LIU Qun;LIU Kun;GAO Xin-yan;ZHU Bing(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700.China;Department of Cardiology,Jining No.1 People’s Hosptial,Jining 272011,Shandong Province)

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]济宁市第一人民医院心内科,山东济宁272011

出　　处：《针刺研究》2020年第4期264-268,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81674085);国家重点研发计划资助项目(No.2018YFC1704600);中央公益性科研院所基本科研业务专项资金(No.ZZZD16001、201814001)。

摘　　要：目的:观察电针"内关"穴对慢性心肌缺血大鼠心交感神经活动及心功能的影响,探讨针灸治疗缺血性心肌病的自主神经机制。方法:SD大鼠随机分为正常组、模型组及电针组,每组8只。采用结扎冠状动脉左前降支法制备慢性心肌缺血大鼠模型。电针组给予双侧"内关"穴电针治疗,20 min/次,1次/d,连续治疗28 d。采用超声心动图观察左心室射血分数(LVEF)及短轴缩短率(LVFS)的变化;采用在体电生理技术观察Ⅱ导联心电图ST段高度、记录大鼠心交感神经活动水平。结果:与正常组比较,模型组大鼠LVEF及LVFS显著下降(P<0.001);与模型组比较,电针组大鼠LVEF及LVFS显著升高(P<0.001,P<0.05)。与正常组比较,模型组大鼠心电图ST段高度显著降低(P<0.01);电针组大鼠心电图ST段高度较模型组显著升高(P<0.05)。与正常组比较,模型组大鼠心交感神经放电频率显著增加(P<0.001);电针组大鼠心交感神经放电频率较模型组显著减少(P<0.01)。结论:电针"内关"穴能够改善慢性心肌缺血大鼠心功能,促进心电图恢复,其机制可能与降低心交感神经异常兴奋,调整交感-副交感平衡有关。Objective To investigate the autonomic nervous mechanism of acupuncture therapy in the treatment of ischemic cardiomyopathy by observing the influence of electroacupuncture(EA) at "Neiguan"(PC6) on the superior cervical cardiac nerve activity and cardiac function in chronic myocardial ischemia(CMI) rats. Methods Male SD rats were randomly divided into control, model and EA groups(n=8 in each group). The CMI model was established by ligating the anterior descending branch of the left coronary artery. EA(15 Hz, 1.5 mA) was applied to bilateral PC6 for 20 min, once a day for 28 consecutive days. Cardiac sympathetic nerve electrical activities(CSNEA), electrocardiogram(ECG) of the standard limb lead Ⅱ and ultrasonic cardiogram(UCG) were recorded for observing changes of ST segment height, left ventricular ejection fraction(LVEF) and left ventricular fractional shortening(LVFS). Results The CSNEA was significantly increased(P<0.001), and the hight of ECG-STⅡ, LVEF and LVFS were considerably decreased in the model group relevant to the control group(P<0.01, P<0.001), while after the intervention, modeling induced increase of CSNEA and decrease of ECG-STⅡ, LVEF and LVFS were obviously suppressed in the EA group in comparison with the model group(P<0.01, P<0.05, P<0.001). Conclusion EA of PC6 can improve cardiac function and myocardial ischemia in CMI rats, which is possibly related to its effect in decreasing cardiac sympathetic hyperactivity.

关 键 词：慢性心肌缺血 电针 超声心动图 心交感神经活动 

分 类 号：R245.97[医药卫生—针灸推拿学]