Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression  

黄花菜改善模拟微重力所致大鼠抑郁的尿液代谢组学研究

作　　者：Teng Xu Yan Wang Cong Lu Li Feng Linxi Fan Jing Sun Bei Fan Qiong Wang Xinmin Liu Fengzhong Wang 许腾;王艳;卢聪;冯利;范琳犀;孙晶;范蓓;王琼;刘新民;王凤忠(中国农业科学院,农产品加工研究所,北京100193;中国医学科学院,北京协和医学院,药用植物研究所,北京100193;巴基斯坦卡拉奇大学,国际化学与生物科学研究院,化学研究所,巴基斯坦卡拉奇752704;巴基斯坦卡拉奇大学,国际化学与生物科学研究院,分子医学与药物研究所,巴基斯坦卡拉奇752705;四川医科大学,四川泸州646000;国家开放大学,北京100081)

机构地区：[1]Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China [3]H.E.J.Research Institute of Chemistry,International Center for Chemical and Biological Sciences,University of Karachi,Karachi-75270,Pakistan [4]Dr.Panjwani Center for Molecular Medicine and Drug Research,International Center for Chemical and Biological Sciences,University of Karachi,Karachi-75270,Pakistan [5]Sichuan Medical University,Luzhou 646000,China [6]The Open University of China,Beijing 100081,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2020年第3期176-191,共16页中国药学（英文版）

基　　金：Chinese Academy of Agricultural Sciences(Grant No.125161015000150013);the grants from the China Agriculture Research System(Grant No.CARS-21).

摘　　要：We investigated the antidepressant-like activity of Hemerocallis citrine Baroni extract(HCE)in a simulated microgravity(SMG)-induced rat model of depression using a metabolomics method.A rat model,generated via 14 d of SMG induction,was validated from the reduced sucrose preference and the enhanced immobility time in the forced swimming test.HCE and paroxetine reversed certain metabolite profiles.Anti-depressant effects of HCE might involve the regulation of several metabolic pathways,such as phenylalanine,glutamic acid,and tryptophan metabolism and changes in energy metabolism.5-Hydroxytryptophan,hippuric acid,phenylacetylglycine,citric acid,3-hydroxykynurenine,cyclic AMP,and L-DOPA profiles were altered upon HCE and paroxetine administration.Furthermore,glutamic acid was only regulated in the HCE group,while xanthurenic acid and deoxyuridine were reversed in the positive group,suggesting differences in the mechanisms between the positive drugs and HCE in improving glutamic acid metabolism.This study provided a theoretical foundation for the application of HCE in depression therapy.本研究基于代谢组学方法研究了黄花菜提取物(HCE)在模拟微重力(SMG)诱导的抑郁症大鼠模型中的抗抑郁作用。通过14天模拟微重力方法建立SMG抑郁大鼠模型,以蔗糖偏爱指数的降低值和强迫游泳试验中不动时间的增加值验证建模成功。研究结果发现HCE和阳性药物帕罗西汀可逆转某些代谢物谱。HCE的抗抑郁作用可能涉及多种代谢途径的调节,例如苯丙氨酸、谷氨酸和色氨酸代谢以及能量代谢的变化。5-羟基色氨酸、柠檬酸、苯乙酰甘氨酸、马尿酸、环腺苷酸和左旋多巴在HCE和帕罗西汀给药后被回调。此外,谷氨酸仅在HCE组中被调节,而黄嘌呤酸和脱氧尿苷水平的波动在阳性组中被回调,这表明阳性药物和HCE在改善谷氨酸代谢中的作用机制可能不同。本研究为HCE在抑郁症治疗中的应用奠定一定的理论基础。

关 键 词：Hemerocallis citrina Anti-depressive Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry Ultra-high performance liquid chromatography coupled to triple-quadrupole mass spectrometry Metabolomics 

分 类 号：R965[医药卫生—药理学]