万古霉素手性膜色谱的研究  

Chiral membrane chromatography based on vancomycin

作　　者：艾萍[1] 杜洋[1] 杨娜[1] 袁黎明[1] AI Ping;DU Yang;YANG Na;YUAN Liming(School of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China)

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《膜科学与技术》2020年第2期1-5,13,共6页Membrane Science and Technology

基　　金：国家自然科学基金地区项目(21365025);国家自然科学基金重大研究计划项目(91856123)。

摘　　要：以聚醚砜膜作为基膜,以万古霉素、1,6-己二异腈酸酯作为单体,利用界面聚合的方法制备了万古霉素-己二异腈酸酯聚醚砜手性高分子复合膜,使用自制的膜色谱装置结合高效液相色谱仪,对手性物质D,L-苯甘氨酸进行了手性膜色谱分离研究.详细研究了流速、流动相、进样量、膜层数、膜尺寸对拆分效果的影响.在优选分离条件下,该手性膜色谱对D,L-苯甘氨酸拆分的分离因子(α)和分离度(Rs)分别为5.66、0.66,该方法的最大优点是在水环境下能对手性化合物进行分离制备,这在色谱的手性分离中是少见的.Using vancomycin and 1,6-diisocyanatehexane as monomers,a chiral composite membrane was prepared by interfacial polymerization on a polyethersulfone support.After the composite membrane was mounted in a home-made device of membrane chromatography for high performance liquid chromatography,the chiral separation of D,L phenylglycine was carried out by the chiral membrane chromatography.By optimizing injection volume,flow rate,constituent of mobile phase,the size of membrane,the layer numbers of membrane,over 5.66 of separation factor(α)and 0.66 of resolution(Rs)could be obtained,respectively.The advantage of the method is able to separate and prepare chiral compounds in an aqueous environment,which is rare in chromatographic chiral separation.

关 键 词：D L苯甘氨酸 万古霉素 手性膜 手性膜色谱 手性分离 

分 类 号：O652.6[理学—分析化学] TQ028.8[理学—化学]