2000—2019年广西狂犬病病毒流行株的基因组遗传稳定性分析  

Genetic stability analysis based on complete genome of wild rabies virus epidemic in Guangxi during 2000-2019

作　　者：覃晴 梁星雪 曹晗 韦显凯 梁晶晶[1,2] 李晓宁 罗廷荣[1,2] QIN Qing;LIANG Xing-xue;CAO Han;WEI Xian-kai;LIANG Jing-jing;LI Xiao-ning;LUO Ting-rong(State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourses,Nanning 530004,China;College of Animal Science and Veterinary Medicine,Guangxi University,Nanning 530004,China;Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China)

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [2]广西大学动物科学技术学院,南宁530004 [3]广西动物疫病预防控制中心,南宁530001

出　　处：《南方农业学报》2020年第3期645-651,共7页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31570147)。

摘　　要：【目的】揭示广西狂犬病病毒(RABV)的流行变异规律及进化特征,为广西狂犬病防控提供科学依据。【方法】对2018年从患狂犬病家犬脑组织分离获得的2株RABV野毒株(GXYZ2018和GXSL2018)进行全基因组扩增与测序,测序结果采用Seq Man进行拼接以获得全基因组序列,并与近20年来的RABV广西流行株进行系统地遗传变异分析。【结果】GXSL2018株和GXYZ2018株的全基因组序列长度均为11923 bp,不同RABV广西流行株间的核苷酸序列同源性在87.1%~99.4%,而2株毒株间的同源性为99.1%;与其他RABV广西流行株相比,GXSL2018株和GXYZ2018株的3’-UTR为69 bp,且在第20位缺失一个核苷酸。基于N基因和G基因核苷酸序列同源性构建的遗传进化树均显示GXSL2018株和GXYZ2018株同属于Group Ⅱ型毒株,且2株毒株N蛋白上的B细胞表面抗原表位(第358~367位氨基酸残基)、Th细胞表位(第404~418位氨基酸残基)和RNA结合域(第298~352位氨基酸残基),以及G蛋白上与病毒毒力密切关联的第333、336、339和357位氨基酸残基及中和抗体相关表位和受体结合位点均高度保守。【结论】GXYZ2018株和GXSL2018株同属于Group Ⅱ型毒株,与近20年来的RABV广西流行株高度相似,病毒蛋白主要功能区的氨基酸序列高度保守,尚未发生变异,全基因组遗传特性稳定。【Objective】To reveal the epidemiological variation and evolution characteristics of rabies virus(RABV) in Guangxi,and provide a scientific basis for the prevention and control of rabies in Guangxi.【Method】The two RABV wild strains(GXYZ2018 and GXSL2018)isolated from the brain tissues of dogs with rabies in 2018 were subjected to complete genome amplification and sequencing. Seq Man soft was used to splice the whole gene sequence,and the results were analyzed systematically for the genetic variation of RABV Guangxi epidemic strains in the past 20 years.【Result】Both GXSL2018 and GXYZ2018 showed 11923 bp full length of the complete genome sequences,and the nucleotide sequence homology with different RABV Guangxi epidemic strains was 87.1%-99.4%,while the homology between the two strains was 99.1%. Compared with other popular RABV strains in Guangxi,the GXSL2018 strain and the GXYZ2018 strain had a 3’-UTR length of 69 bp and a nucleotide deletion at position 20. The genetic evolutionary trees constructed based on the nucleotide sequence homology of the N gene and the G gene both showed that the GXSL2018 strain and the GXYZ2018 strain belonged to the Group Ⅱ type strain.And the B cell surface epitopes(amino acid residues358-367),Th cell epitopes(amino acid residues 404-418)and RNA binding domains(amino acids 298-352)on the N protein of the two strains and amino acid residues 333,336,339 and 357,which were closely related to viral virulence,antibody-related epitopes,and receptor binding sites on the G protein were highly conserved.【Conclusion】The GXYZ2018 strain and the GXSL2018 strain belong to the Group Ⅱ type,which are highly similar to the RABV Guangxi epidemic strains in the past 20 years.The amino acid sequence of the main functional region of the viral protein is highly conserved,no mutation has occurred,and the whole genome inheritance characteristics arestable.

关 键 词：狂犬病病毒(RABV) 基因组 遗传稳定性 GroupⅡ型毒株 

分 类 号：S852.659.5[农业科学—基础兽医学]