温阳消饮方对慢性心力衰竭大鼠PI3K-Akt信号通路表达及相关凋亡蛋白的影响  

Effects of Wenyang Xiaoyin Formula on the expression of PI3K-Akt signal pathway and related apoptotic proteins in chronic heart failure rats

作　　者：杨嘉豪 崔瑞琴[1] 周波[1] 李卫强[1] 马立旭[2] 徐建虎[1] YANG Jia-hao;CUI Rui-qin;ZHOU Bo;LI Wei-qiang;MA Li-xu;XU Jian-hu(College of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,China;Department of Medical Record Statistics,Ningxia People’s Hospital,Yinchuan 750002,China)

机构地区：[1]宁夏医科大学中医学院,银川750004 [2]宁夏回族自治区人民医院病案统计科,银川750002

出　　处：《中华中医药杂志》2020年第1期145-149,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：宁夏回族自治区自然科学基金项目(No.NZ16079)。

摘　　要：目的:探讨温阳消饮方治疗慢性心力衰竭的可能作用机制。方法:将50只SD大鼠随机取10只为空白组,其余均为造模组。造模组采用腹腔注射阿霉素诱导慢性心力衰竭模型,造模结束后采用多普勒超声心动图机测定大鼠心功能,造模成功后将大鼠随机分为模型组、温阳消饮组、血府逐瘀组、美托洛尔组,每组10只,分别进行干预。治疗后采用HE染色法观察大鼠心肌病理形态学变化,Western Blot法检测心肌组织中p-PI3K、p-Akt蛋白含量,免疫组织化学法检测心肌组织Bcl-2、Bax及Caspase-8表达情况。结果:与空白组比较,模型组大鼠心肌组织p-PI3K、p-Akt、Bcl-2蛋白表达及Bcl-2/Bax值下降(P<0.01),Bax、Caspase-8蛋白表达升高(P<0.05,P<0.01);与模型组比较,各给药组大鼠心肌组织p-PI3K、p-Akt、Bcl-2蛋白表达及Bcl-2/Bax值升高(P<0.01),Bax、Caspase-8蛋白表达降低(P<0.05,P<0.01)。结论:温阳消饮方可能通过调控PI3K-Akt信号通路及相关凋亡蛋白的表达,从而发挥保护心肌的作用。Objective:To investigate the possible mechanism of Wenyang Xiaoyin Formula in treating chronic heart failure.Methods:A total of 50 SD rats were randomly divided into control group (n=10) and CHF group (n=40).The rat model of chronic heart failure was reproduced by intraperitoneal injection of adriamycin.After the completion of the model making,Doppler echocardiography was used to determine the cardiac function of rats and randomly divided into model group,Wenyang Xiaoyin group,Xuefu Zhuyu group and metoprolol group,with 10 rats in each group.After treatment,HE staining was used to observe the changes of myocardial pathological morphology in rats.The protein contents of p-PI3K,p-Akt were detected by Western Blot.The expression of Bcl-2 and Bax and Caspase-8 was detected by immunohistochemistry.Results:Compared with the control group,p-PI3K,p-Akt,Bcl-2 expressions in the myocardial tissue and Bcl-2/Bax of the model group were decreased (P<0.01),the expressions of Bax and Caspase-8 were increased (P<0.05,P<0.01).Compared with the model group,the expressions of p-PI3K,p-Akt,Bcl-2 and Bcl-2/Bax were increased in treatment groups (P<0.01),and the expressions of Bax,Caspase-8 were decreased (P<0.05,P<0.01).Conclusion:The yang-warming and fluid retention-resolving method may play a role in protecting myocardium by regulating the expression of PI3K-Akt signaling transduction pathway and related apoptotic proteins.

关 键 词：温阳消饮 慢性心力衰竭 PI3K-AKT信号通路 BAX BCL-2 CASPASE-8 

分 类 号：R285.5[医药卫生—中药学]