去卵巢大鼠全身肌肉、脂肪和雌二醇含量与骨密度的相关性及补肾健脾穴位针刺的调节作用  

Correlation between muscle, fat, estradiol levels and bone mineral density and regulation of acupuncture at tonifying kidney and invigorating spleen in ovariectomized rats

作　　者：原淳淳 艾尔扎提·艾尔宝 王达宝 杨飞宇 张云龙 赵英侠 YUAN Chun-chun;AIERZHATI Ai-er-bao;WANG Da-bao;YANG Fei-yu;ZHANG Yun-long;ZHAO Ying-xia(Basic Medical College of Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海中医药大学基础医学院组织胚胎学教研室,上海201203

出　　处：《中华中医药杂志》2020年第1期434-436,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家基础科学人才培养基金项目(No.J1103607);上海市大学生科创项目(No.2016KCZ08);上海市教委预算内科研项目(No.08cz01);上海市教委第五期重点学科项目(No.J50301)。

摘　　要：目的:探讨绝经后骨质疏松症的发生与全身肌肉、脂肪变化的关系及针刺的作用。方法:6月龄雌性SD大鼠去除双侧卵巢后第4天,取补肾健脾、通经活络穴位肾俞、脾俞、足三里(均双侧)及大椎针刺3个疗程,以骨密度检测仪prodigy测定全身骨密度(BMD)、肌肉和脂肪含量,以ELISA法测定血清雌二醇(E2)含量,以Western Blot技术测定双侧股四头肌肌球蛋白重链(MHC)蛋白表达水平,以假手术组和模型组作为对照。结果:与假手术组比较,模型组大鼠全身BMD和肌肉含量呈下降趋势,脂肪与E2含量明显升高(P<0.05),MHC蛋白呈高表达趋势;与模型组比较,针刺组全身BMD和肌肉含量呈上升趋势,脂肪与E2含量明显降低(P<0.01,P<0.05),MHC蛋白呈低表达(P<0.05)。结论:绝经后骨质疏松症的发生与肌肉减少、脂肪增加密切相关,针刺具有良性调节作用。Objective:To explore the relationship between the occurrence of postmenopausal osteoporosis and changes in muscle and fat and the effects of acupuncture.Methods:On the 4th day after the removal of bilateral ovaries in 6-month-old female SD rats,taking 3 courses of tonifying the kidney and invigorating the spleen and promoting menstruation acupuncture points:‘Shenshu (BL23) ’,‘Pishu (BL20)’,‘Zusanli (ST36)’ (both bilateral) and ‘Dazhui (GV14)’ acupuncture.The BMD,muscle and fat content of the whole body were measured by bone densitometer prodigy.Serum E2 content was determined by ELISA,and the expression of MHC protein in bilateral quadriceps muscle was determined by Western Blot.The sham operation group and the model group were used as controls.Results:Compared with the sham operation group,the content of BMD and muscle in the model group showed a downward trend,and the fat and E2 levels increased significantly (P<0.05),and the MHC protein was highly expressed.Compared with the model group,the content of BMD and muscle in the acupuncture group increased,the content of fat and E2 decreased (P<0.01,P<0.05),MHC was low in expression (P<0.05).Conclusion:The occurrence of postmenopausal osteoporosis is closely related to muscle loss and fat increase,and acupuncture has a benign regulatory effect.

关 键 词：骨质疏松症 去势大鼠 补肾健脾 针刺 骨肉不相亲 肌肉 脂肪 骨密度 

分 类 号：R245[医药卫生—针灸推拿学]