电针对高脂饮食诱导的胰岛素抵抗大鼠海马胰岛素降解酶、PPARγ、β-catenin与Aβ42表达的影响  

Effects of electro-acupuncture on the expression of IDE,PPARγ,β-catenin and Aβ42 in hippocampus of insulin resistance rats induced by high-fat diet

作　　者：王静芝 陈丽 徐芬 瞿涛 杜艳军 WANG Jing-zhi;CHEN Li;XU Fen;QU Tao;DU Yan-jun(College of Acupuncture Moxibustion and Orthopaedics,Hubei University of Chinese Medicine,Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion,Wuhan 430061,China;The General Hospital of Wuhan,Wuhan 430014,China)

机构地区：[1]湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心,武汉430061 [2]武汉市中心学院,武汉430014

出　　处：《中华中医药杂志》2020年第2期642-645,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81473786,No.81603199);湖北省自然科学基金项目(No.2018CFB351,No.2017CFB184);湖北中医药大学校级课题(No.XJ2014KJ004)。

摘　　要：目的:观察电针对胰岛素抵抗大鼠海马胰岛素降解酶(IDE)、过氧化物酶体增殖物激活受体-γ(PPAR-γ)、β连环蛋白(β-catenin)与β淀粉样蛋白(Aβ42)表达的影响。方法:40只Wistar大鼠随机分为正常组、模型组、预防组和电针组,每组10只。电针治疗后,免疫组织化学法检测大鼠海马Aβ42阳性表达;实时荧光定量PCR检测大鼠海马IDE、PPARγ和β-catenin表达。结果:与正常组比较,其他各组大鼠海马Aβ42的阳性表达显著升高(P<0.01,P<0.05),IDE、PPARγ与β-catenin表达显著减少(P<0.01,P<0.05);与模型组比较,预防组、电针组大鼠海马Aβ42阳性表达显著减少(P<0.01),IDE、PPARγ和β-catenin表达显著增加(P<0.05,P<0.01);与预防组比较,电针组大鼠海马IDE、PPARγ表达显著减少(P<0.05)。结论:电针降低胰岛素抵抗大鼠海马Aβ42阳性表达,其作用机制可能与电针增加IDE、PPARγ、β-catenin表达相关。Objective:To observe the effects of electro-acupuncture on the expression of IDE,PPARγ,β-catenin and Aβ42 in hippocampus of insulin resistance rats.Methods:Forty Wistar rats were divided into 4 groups:normal group,model group,pre-EA group and EA group,10 rats each group.Expression levels of Aβ42 in hippocampus of IR rats;The expression of IDE,PPARγandβ-catenin mRNA in hippocampus were analyzed by real-time fluorescence quantitative PCR.Results:Compared with normal group,in other three groups,the expression levels of Aβ42 in hippocampus increased(P<0.01,P<0.05),the expression of IDE,PPARγandβ-catenin mRNA in hippocampus decreased(P<0.01,P<0.05)in other 3 groups;Compared with model group,in pre-EA group and EA group,the expression levels of Aβ42 in hippocampus decreased(P<0.01),the expression of IDE,PPARγandβ-catenin mRNA in hippocampus increased(P<0.05,P<0.01)in pre-EA group and EA group.Compared with pre-EA group,the expression of IDE,PPARγmRNA in hippocampus decreased(P<0.05)in EA group.Conclusion:The mechanism of electro-acupuncture improvement on expression levels of Aβ42 in insulin resistance model rats,is pertaining to the regulation of regulating the expression of IDE,PPARγandβ-catenin mRNA in hippocampus.

关 键 词：电针治疗 胰岛素抵抗 β淀粉样蛋白 胰岛素降解酶 过氧化物酶体增殖物激活受体-Γ Β连环蛋白 

分 类 号：R245[医药卫生—针灸推拿学]