马钱子碱对人结肠癌HT-29细胞线粒体膜电位及凋亡的影响  

Effects of brucine on colon cancer cell HT-29 in mitochondrial membrane potential and apoptosis

作　　者：杨珍 冯振宇[1] 孟霜[1] 马小娟 周晓荣 赵建平[1] YANG Zhen;FENG Zhen-yu;MENG Shuang;MA Xiao-juan;ZHOU Xiao-rong;ZHAO Jian-ping(Shanxi Hospital of Integrated Traditional and Western Medicine,Shanxi University of Chinese Medicine,Taiyuan 030013,China;Shanxi University of Chinese Medicine,Taiyuan 030000,China)

机构地区：[1]山西中医学院中西医结合医院,太原030013 [2]山西中医药大学,太原030000

出　　处：《中华中医药杂志》2020年第2期950-952,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家国际科技合作专项项目(No.2014DFA33150)。

摘　　要：目的:观察马钱子碱诱导人结肠癌HT-29细胞线粒体膜电位变化及凋亡的作用。方法:以人结肠癌HT-29细胞为研究对象,用不同浓度的马钱子碱作用于HT-29细胞,Annexin V-FITC/PI双标法检测细胞凋亡率;流式细胞仪检测细胞线粒体膜电位变化。结果:马钱子碱可诱导HT-29细胞凋亡(P<0.01);同时可降低线粒体膜电位(P<0.01)。结论:马钱子碱可诱导人结肠癌HT-29细胞凋亡,其机制可能与其降低线粒体膜电位有关。Objective:To explore the effect of brucine in inducing apoptosis and mitochondrial membrane potential change of colon cancer cell HT-29 and its mechanism.Methods:In the study,colon cancer cell HT-29 was taken as the experimental subject and processed with different concentrations of brucine.The apoptosis rate of HT-29 cells was determined by Annexin FITC/PI double staining.The changes in mitochondrial membrane potential were measured by the flow cytometry.Results:The results showed that brucine significantly induced the apoptosis on HT-29 cells(P<0.01),and notably reduced mitochondrial membrane potential(P<0.01).Conclusion:Brucine can induce the apoptosis of HT-29 cells,and its mechanism may be related to the changes of mitochondrial membrane potential.

关 键 词：马钱子碱 细胞凋亡 线粒体膜电位 HT-29细胞 

分 类 号：R285[医药卫生—中药学]