蓖麻赤霉素受体蛋白RcGID1B的生物信息学分析  

Bioinformatics Analysis of Castor Gibberellin Receptor Protein RcGID1B

作　　者：赵秀平 李国瑞[1,2] 风兰 韩雯毓 王双 孙佳欣 闫星伊 黄凤兰[1,2] 陈永胜[1,2] Zhao Xiuping;Li Guorui;Feng Lan;HanWenyu;Wang Shuang;Sun Jiaxin;Yan Xingyi;Huang Fenglan;Chen Yongsheng(College of Life Science,Inner Mongolia University for Nationalities,Tongliao,028043;Key Laboratory for Castor Breeding of Inner Mongolia,Inner Mongolia Industrial Engineering Research Center of Universities for Castor,Inner Mongolian Collaborative Innovation Center for Castor Industry,Inner Mongolia University for Nationalities,Tongliao,028043)

机构地区：[1]内蒙古民族大学生命科学学院,通辽028043 [2]内蒙古民族大学,内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古自治区蓖麻产业协同创新中心,内蒙古自治区蓖麻育种重点实验室,通辽028043

出　　处：《分子植物育种》2020年第8期2497-2502,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31460353);内蒙古自治区科技创新引导资金项目(KJCX15002);内蒙古民族大学科研项目(MDK2016008,MDK2017034,MDK2017035);草原英才创新团队项目;国家青年科学基金(31401418)共同资助.

摘　　要：赤霉素受体GID1(gibberellin insensitive dwarf1,GID1)是一种可溶性蛋白,是赤霉素信号转导途径中与赤霉素结合的关键蛋白。根据KEGG数据库得到的蓖麻赤霉素受体蛋白RcGID1B氨基酸序列并对其进行生物信息学分析。结果表明:RcGID1B是由345个氨基酸组成的不稳定亲水蛋白,等电点为6.42,无跨膜结构,含组氨酸和丝氨酸活性位点,有Abhydrolase-3保守结构域,属于Abhydrolase家族。进化树聚类分析显示蓖麻与同为大戟科植物的木薯、橡胶树和麻风树聚类到了一起,符合生物学分类。本实验对RcGID1B进行生物信息学分析,旨在为蓖麻赤霉素信号转导途径的研究和蓖麻矮化研究提供参考基础。Gibberellin receptor GID1(gibberellin insensible dwarf1,GID1)is a soluble protein and a key protein that binds gibberellin in gibberellin signal transduction pathway.Bioinformatics analysis of castor Gibberellin receptor rrotein RcGID1B was carried out according to amino acid sequence of RcGID1B obtained from KEGG database.The results showed that RcGID1B is an unstable hydrophilic protein composed of 345 amino acids,with isoelectric point of 6.42,no transmembrane structure,histidine and serine active sites,and Abhydrase-3 conserved domain,belonging to the Abhydrase family.Phylogenetic tree cluster analysis showed that castor and cassava,rubber tree and jatropha of Euphorbiaceae were clustered together,which accorded with biological classification.The bioinformatics analysis of RcGID1B was carried out in this experiment,aiming to provide a reference basis for the research of gibberellin signal transduction pathway and dwarfing research of castor.

关 键 词：蓖麻(Ricinus communis L.) 赤霉素受体 RcGID1B 生物信息学分析 

分 类 号：S565.6[农业科学—作物学]