山豆根基因组DNA高效提取方法的建立与优化  

Development and Optimization of an Efficient Extraction Method of DNA from Sophora tonkinensis

作　　者：占塔鹏 黄舒婷 乔柱[2] 羽健宾 韦莉 何龙飞[1] 肖冬[1,2] Chanthaphoone Keovongkod;Huang Shuting;Qiao Zhu;Yu Jianbin;Wei Li;He Longfei;Xiao Dong(College of Agriculture,Guangxi University,Nanning,530004;Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plants,Nanning,530023)

机构地区：[1]广西大学农学院,南宁530005 [2]广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,南宁530023

出　　处：《分子植物育种》2020年第8期2585-2590,共6页Molecular Plant Breeding

基　　金：广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室开放性研究项目(201601);广西自然科学基金(2017GXNSFAA198252;2016GXNSFBA380223)共同资助。

摘　　要：为了探寻优化山豆根DNA提取的方法,本研究以山豆根的叶片为材料,用3种改良的CTAB法提取山豆根DNA,并将提取的DNA经分光光度计和电泳检测,用于cpDNA引物筛选试验,找出最有效的山豆根DNA提取方法。结果显示,高PVP和β-巯基乙醇提取法和样品预处理法可用于提取山豆根基因组DNA。其中,样品预处理法山豆根DNA的效果最好,提取DNA浓度高、质量好,引物筛选结果条带清晰且单一。本研究建立了山豆根高质量的DNA提取方法,为进行分子生物学研究提供支持。In order to optimize the method of extracting DNA from the root of Sophora tonkinensis,three improved CTAB methods were used to extract the DNA,from the leaves of Sophora tonkinensis.The extracted DNA was detected by spectrophotometer and electrophoresis,and was used in cpDNA primer screening experiment to find out the most effective method for DNA extraction from Sophora tonkinensis.The results showed that high level of PVP andβ-ME extraction method and sample pretreatment extraction method could be used to extract the genomic DNA of Sophora tonkinensis.Among them,the effect of sample pretreatment was the best,with high concentration and quality of extracted DNA and clear and single primer screening results.In this study,a high-quality DNA extraction method for extracting genomic DNA from Sophora tonkinensis was established to provide support for molecular biology research.

关 键 词：山豆根(Sophora tonkinensis) 基因组DNA提取 改良CTAB 法 

分 类 号：S567.19[农业科学—中草药栽培]