不同品种藜麦苗期对海拔变化的生理响应  

Effects of Different Altitude on Seedling Growth and Physiological Characteristics of Chenopodium quinoa

作　　者：何斌 刘文瑜[2] 王旺田[1] 杨发荣[2] 黄杰[2] 魏玉明[2] 郑凯翔 He Bin;Liu Wenyu;WangWangtian;Yang Farong;Huang Jie;Wei Yuming;Zheng Kaixiang(Gansu Provincial Key Laboratory of Arid Land Crop Science,College of Life Science and Technology,Gansu Agricultural University,Lanzhou,730070;Institute of Pasture and Green Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou,730070)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃省干旱生境作物学重点试验室,兰州730070 [2]甘肃省农业科学院,畜草与绿色农业研究所,兰州730070

出　　处：《分子植物育种》2020年第8期2702-2712,共11页Molecular Plant Breeding

基　　金：国家自然基金(31660357);甘肃农业大学青年导师扶持基金(GAU-QDFC-2018-12);甘肃省科技厅重点研发项目(18YF1WA093);甘肃省农业科学院农业科技创新专项计划(2017GASS66)共同资助。

摘　　要：为研究不同品种藜麦幼苗对海拔梯度变化的生理响应机制,本试验以4个藜麦品种—‘陇藜1号’、‘陇藜2号’、‘陇藜3号’及‘陇藜4号’为材料,选取甘南州临潭县3个不同海拔(2380 m,2580 m,2780 m)分布区为试验地。通过测定幼苗生物量及生理生化指标,分析不同藜麦品种幼苗在不同海拔试验区的生理响应机制,为建立藜麦品种和环境相适宜的评价体系提供参考依据。结果表明,随海拔升高,藜麦叶片叶绿素、可溶性蛋白以及抗坏血酸过氧化物酶(APX)含量逐渐降低,海拔2780 m最低;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)逐渐升高,在海拔2780 m达到最大值;超氧阴离子(O2·-)产生速率呈现先升高后降低的趋势。不同藜麦品种幼苗生物量随着海拔的升高逐渐降低。说明高海拔下太阳辐射强度增大、温度降低,导致藜麦幼苗植株抗氧化酶活性升高以清除多余活性氧(ROS)自由基,使得植株能够适应高海拔环境而正常生长,但生物量较低海拔仍有降低。因此,不同品种藜麦在低海拔地区受到的胁迫较低,虽然在高海拔区域胁迫加剧,但藜麦幼苗可以正常生长并进行干物质的积累。In order to research the physiological response mechanism to altitude gradient changes of different varieties of quinoa seedlings,in this experiment,four varieties of quinoa,LongLi-1,LongLi-2,LongLi-3 and Long-Li-4 were used as materials,and three different altitudes(2380 m,2580 m,2780 m)in Lintan County,Gannan Prefecture were selected as experimental sites.By measuring biomass,physiological and biochemical indicators,of seedlings,the physiological response mechanism of different varieties of quinoa seedlings at different altitudes was analyzed,which provides reference for the establishment of a suitable evaluation system of quinoa variety and environment.The results showed that the contents of chlorophyll,soluble protein and ascorbic acid peroxidase(APX)in quinoa leaves decreased gradually with the increase of altitude,reaching the lowest value at 2780 m.Superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)gradually increased,reaching the maximum at 2780 m.The production rate of superoxide anion(O2·-)increased first and then decreased.With the increase of altitude,the cumulative biomass of seedlings of different quinoa varieties decreased gradually.The results showed that the increase of solar radiation intensity and the decrease of temperature at high altitude led to the increase of antioxidant enzymes activity in quinoa seedlings to eliminate excess reactive oxygen species(ROS)free radicals,which enabled the plants to adapt to the high altitude environment and grow normally,but the biomass at lower altitude still decreased in varying degrees.In this study,the reason for the difference between the cumulative changes of dry matter and the physiological and biochemical changes of quinoa seedlings lies in the different degree of stress received by the three groups of materials,that is the materials in low altitude areas are under lower stress,with the increase of altitude,the stress intensifies,but quinoa seedlings can germinate normally and accumulate dry matter.

关 键 词：藜麦(Chenopodium quinoa) 海拔 抗氧化酶 相关性分析 

分 类 号：S519[农业科学—作物学]