参麻益智方对多发脑梗血管性痴呆大鼠认知功能和脑白质损伤的影响  

Effect of Shenmayizhi Decoction on Cognitive Function and White Matter Damage in Multiple Cerebral Infarction VD Rats

作　　者：李泽惠 曹宇[1] 刘佳妮[1] 刘美霞[1] 裴卉[1] 李浩[1] LI Zehui;CAO Yu;LIU Jiani;LIU Meixia;PEI Hui;LI Hao(Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China)

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《世界中医药》2020年第8期1120-1123,1128,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(81603616);北京市科技计划项目(Z171100001717016);中国中医科学院院级自主选题(ZZ11-025)。

摘　　要：目的:研究参麻益智方对血管性痴呆(VD)模型大鼠认知功能和脑白质损伤的影响。方法:将60只Wistar大鼠经造模手术后随机分为假手术组、模型组、参麻益智方低剂量组、参麻益智方中剂量组、参麻益智方高剂量组和多奈哌齐组。给药2周后行水迷宫实验检测大鼠的认知功能,脑组织LFB染色观察胼胝体髓鞘脱失情况并进行脑白质损伤评分,Western blot法检测MBP蛋白表达。结果:参麻益智方高剂量组大鼠水迷宫逃避潜伏期明显缩短,目标象限停留时间明显延长,脑白质损伤评分明显降低,MBP表达水平明显升高。结论:参麻益智方可以改善VD大鼠的认知功能,减轻VD大鼠的脑白质损伤。Objective:To study the effect of Shenma Yizhi Decoction(SMYZD)on cognitive function and white matter lesions in VD rats.Methods:A total of 60 surgery modeled Wistar rats were randomly divided into Sham group,Model group,SMYZD-L group,SMYZD-M group,SMYZD-H group and Donepezil group.After 2 weeks administration of SMYZD,Morris water maze test was used to detect cognitive function of rats.Brain tissue LFB staining was used to observe loss of myelin sheath of the Corpus callosum and White matter damage scoring.Western blot was used to detect the expression of myelin basic protein(MBP).Results:Escape latency of rats in SMYZD-H group significantly shortened.Time spent in target quadrant significantly prolonged.Grading score of white matter damage significantly diminished,and MBP expression augmented.Conclusion:Shenma Yizhi Decoction could improve cognitive dysfunction and alleviate white matter damage of VD rats.

关 键 词：血管性痴呆 多发脑梗 参麻益智方 脑白质 髓鞘 

分 类 号：R285.5[医药卫生—中药学]