单细胞拉曼分选技术在大肠杆菌分离中的应用  

Application of Single Cell Raman Sorting Technique in Separation of Escherichia coli

作　　者：李备 张来明[1] 李文杰[1,2] Li Bei;Zhang Lai-ming;Li Wen-jie(Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Jilin Changchun 130033;HOOKE Instruments Ltd.,Jilin Changchun 130033)

机构地区：[1]中国科学院长春光学精密机械与物理研究所,吉林长春130033 [2]长春长光辰英生物科学仪器有限公司,吉林长春130033

出　　处：《生物化工》2020年第2期75-77,共3页Biological Chemical Engineering

摘　　要：单细胞拉曼光谱(SCRS)是固有的生化特征和单细胞的“化学指纹”,可用于表征细胞的表型变化、生理状态和功能。本文在研究SCRS的基础上,结合单细胞分选技术提出了一种无标记的细胞分选方法,实现了无标记、无损精准分离单细胞,并通过这一机理,实现大肠杆菌的拉曼识别和精准分离,建立了完整的实验体系。Singlecell Raman spectroscopy(SCRS)is an intrinsic biochemical feature and a single cell“chemical fingerprint”that can be used to characterize phenotypic changes,physiological states,and functions of cells.On the basis of SCRS,combined with single-cell sorting technology,this paper proposed an unlabeled cell sorting method,realizing unlabeled and lossless accurate separation of single cells,and through this mechanism,realizing the Raman recognition and accurate separation of E.coli,and establishing a complete experimental system.

关 键 词：拉曼识别 单细胞分选 精准分离 

分 类 号：O657.37[理学—分析化学]